Toxicity of silver nanoparticles in mouse bone marrow-derived dendritic cells

Implications for phenotype

Sandra Castro-Gamboa, Maritza Roxana Garcia-Garcia, Gabriela Piñon-Zarate, Marcela Rojas-Lemus, Katia Jarquin-Yañez, Miguel Angel Herrera-Enriquez, Teresa I. Fortoul, Yanis Toledano-Magaña, Trinidad Garcia-Iglesias, Alexey Pestryakov, Andres Eliu Castell-Rodriguez, Nina Bogdanchikova

Результат исследований: Материалы для журналаСтатья

1 цитирование (Scopus)

Выдержка

Silver nanoparticles (AgNP) are one of the most studied nanoparticles due to their anti-bacterial, -fungal, -viral, -parasitic, and -inflammatory properties. This raises the need to evaluate the toxicity and biological effects of AgNP in the immune system in order to develop new safer biomedical products. In this study, an AgNP formulation currently approved for veterinary applications was applied to mouse bone marrow-derived dendritic cells (BMDC), considered important antigen-presenting cells of the immune system, to evaluate cytotoxicity, genotoxicity, and any significant influence on expression of cellular markers associated with BMDC phenotype and maturation status. The results showed that after 12 h of AgNP exposure, a significant decrease in BMDC viability occurred at the highest concentration tested (1.0 µg AgNP/ml) and at lower doses, the cells maintained membrane integrity and metabolic activity. DNA damage was not significant with any AgNP level aside from the 1.0 µg AgNP/ml level. Regarding phenotype, no differences in expression of CD40 (co-stimulatory molecule highly present in mature BMDC) or in CD273 (a marker for inhibitory T-cell response) were observed. The current results showed that the toxicity of this AgNP formulation was dose-related. The findings also suggest BMDC could maintain structural conservation of co-stimulatory/co-inhibitory surface molecules after 12 h of exposure to this AgNP. This work represents the first step in identifying the toxic effects of this AgNP formulation on dendritic cells.

Язык оригиналаАнглийский
ЖурналJournal of Immunotoxicology
DOI
СостояниеОпубликовано - 1 янв 2019

Отпечаток

Silver
Nanoparticles
Dendritic Cells
Toxicity
Bone
Bone Marrow
Phenotype
Immune system
Immune System
Molecules
T-cells
Poisons
Antigen-Presenting Cells
Cell membranes
Cytotoxicity
DNA Damage
Conservation
Cell Survival
Cell Membrane
T-Lymphocytes

ASJC Scopus subject areas

  • Immunology
  • Toxicology

Цитировать

Castro-Gamboa, S., Garcia-Garcia, M. R., Piñon-Zarate, G., Rojas-Lemus, M., Jarquin-Yañez, K., Angel Herrera-Enriquez, M., ... Bogdanchikova, N. (2019). Toxicity of silver nanoparticles in mouse bone marrow-derived dendritic cells: Implications for phenotype. Journal of Immunotoxicology. https://doi.org/10.1080/1547691X.2019.1584652

Toxicity of silver nanoparticles in mouse bone marrow-derived dendritic cells : Implications for phenotype. / Castro-Gamboa, Sandra; Garcia-Garcia, Maritza Roxana; Piñon-Zarate, Gabriela; Rojas-Lemus, Marcela; Jarquin-Yañez, Katia; Angel Herrera-Enriquez, Miguel; Fortoul, Teresa I.; Toledano-Magaña, Yanis; Garcia-Iglesias, Trinidad; Pestryakov, Alexey; Eliu Castell-Rodriguez, Andres; Bogdanchikova, Nina.

В: Journal of Immunotoxicology, 01.01.2019.

Результат исследований: Материалы для журналаСтатья

Castro-Gamboa, S, Garcia-Garcia, MR, Piñon-Zarate, G, Rojas-Lemus, M, Jarquin-Yañez, K, Angel Herrera-Enriquez, M, Fortoul, TI, Toledano-Magaña, Y, Garcia-Iglesias, T, Pestryakov, A, Eliu Castell-Rodriguez, A & Bogdanchikova, N 2019, 'Toxicity of silver nanoparticles in mouse bone marrow-derived dendritic cells: Implications for phenotype', Journal of Immunotoxicology. https://doi.org/10.1080/1547691X.2019.1584652
Castro-Gamboa S, Garcia-Garcia MR, Piñon-Zarate G, Rojas-Lemus M, Jarquin-Yañez K, Angel Herrera-Enriquez M и соавт. Toxicity of silver nanoparticles in mouse bone marrow-derived dendritic cells: Implications for phenotype. Journal of Immunotoxicology. 2019 Янв. 1. https://doi.org/10.1080/1547691X.2019.1584652
Castro-Gamboa, Sandra ; Garcia-Garcia, Maritza Roxana ; Piñon-Zarate, Gabriela ; Rojas-Lemus, Marcela ; Jarquin-Yañez, Katia ; Angel Herrera-Enriquez, Miguel ; Fortoul, Teresa I. ; Toledano-Magaña, Yanis ; Garcia-Iglesias, Trinidad ; Pestryakov, Alexey ; Eliu Castell-Rodriguez, Andres ; Bogdanchikova, Nina. / Toxicity of silver nanoparticles in mouse bone marrow-derived dendritic cells : Implications for phenotype. В: Journal of Immunotoxicology. 2019.
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abstract = "Silver nanoparticles (AgNP) are one of the most studied nanoparticles due to their anti-bacterial, -fungal, -viral, -parasitic, and -inflammatory properties. This raises the need to evaluate the toxicity and biological effects of AgNP in the immune system in order to develop new safer biomedical products. In this study, an AgNP formulation currently approved for veterinary applications was applied to mouse bone marrow-derived dendritic cells (BMDC), considered important antigen-presenting cells of the immune system, to evaluate cytotoxicity, genotoxicity, and any significant influence on expression of cellular markers associated with BMDC phenotype and maturation status. The results showed that after 12 h of AgNP exposure, a significant decrease in BMDC viability occurred at the highest concentration tested (1.0 µg AgNP/ml) and at lower doses, the cells maintained membrane integrity and metabolic activity. DNA damage was not significant with any AgNP level aside from the 1.0 µg AgNP/ml level. Regarding phenotype, no differences in expression of CD40 (co-stimulatory molecule highly present in mature BMDC) or in CD273 (a marker for inhibitory T-cell response) were observed. The current results showed that the toxicity of this AgNP formulation was dose-related. The findings also suggest BMDC could maintain structural conservation of co-stimulatory/co-inhibitory surface molecules after 12 h of exposure to this AgNP. This work represents the first step in identifying the toxic effects of this AgNP formulation on dendritic cells.",
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AU - Rojas-Lemus, Marcela

AU - Jarquin-Yañez, Katia

AU - Angel Herrera-Enriquez, Miguel

AU - Fortoul, Teresa I.

AU - Toledano-Magaña, Yanis

AU - Garcia-Iglesias, Trinidad

AU - Pestryakov, Alexey

AU - Eliu Castell-Rodriguez, Andres

AU - Bogdanchikova, Nina

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