Three Structural Motives of the Rbp10 Minisubunit of Nuclear RNA Polymerases Are Strictly Conserved between Eukaryotes

G. V. Shpakovskii, E. N. Lebedenko

Результат исследований: Материалы для журналаСтатьярецензирование

Аннотация

The rpb10+ cDNA from the fission yeast Schizosaccharomyces pombe was cloned using two independent approaches (PCR and genetic suppression). The cloned cDNA encoded the Rpb10 subunit common for all three RNA polymerases. Comparison of the deduced amino acid sequence of the Sz. pombe Rbp10 subunit (71 amino acid residues) with those of the homologous subunits of RNA polymerases I, II, and III from Saccharomyces cerevisiae and Homo sapiens revealed that heptapeptides RCFT/SCGK (residues 6-12), RYCCRRM (residues 43-49), and HVDLIEK (residues 53-59) were evolutionarily the most conserved structural motifs of these subunits. It is shown that the Rbp10 subunit from Sz. pombe can substitute its homolog (ABC10β) in the baker's yeast S. cerevisiae.

Язык оригиналаАнглийский
Страницы (с-по)x11-940
ЖурналBioorganicheskaya Khimiya
Том22
Номер выпуска12
СостояниеОпубликовано - 1996
Опубликовано для внешнего пользованияДа

ASJC Scopus subject areas

  • Medicine(all)

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