TY - JOUR
T1 - The voltammetric determination of peroxynitrite at a mercury film electrode
AU - Zakharova, Elza A.
AU - Yurmazova, Tatyana A.
AU - Nazarov, Boris F.
AU - Wildgoose, Gregory G.
AU - Compton, Richard G.
PY - 2007/3/12
Y1 - 2007/3/12
N2 - This report describes the direct voltammetric detection of peroxynitrite (PN, ONOO-), an important analyte in many physiological biochemical pathways, at a mercury film electrode (MFE) at alkaline pH. The voltammetric response of PN is observed as a cathodic inverted peak when the potential is swept in the oxidative direction at ca. -0.1 V vs. a Ag/AgCl/1 M KCl reference electrode. An mechanism is proposed to explain this behaviour involving the initial formation of a thin film of Hg(i) oxide on the electrode which is then oxidised by PN to form HgO in a chemical step. The HgO is then reduced back to Hg(0) at the electrode potential of interest resulting in a net cathodic current. The parameters affecting the voltammetry of PN at a MFE, including the concentration of PN (10-5-10-4 M), the effect of varying scan rate (5-100 mV s-1) and pH are investigated and the kinetics of PN decay in alkaline solutions ranging from pH 9-13 are explored electrochemically.
AB - This report describes the direct voltammetric detection of peroxynitrite (PN, ONOO-), an important analyte in many physiological biochemical pathways, at a mercury film electrode (MFE) at alkaline pH. The voltammetric response of PN is observed as a cathodic inverted peak when the potential is swept in the oxidative direction at ca. -0.1 V vs. a Ag/AgCl/1 M KCl reference electrode. An mechanism is proposed to explain this behaviour involving the initial formation of a thin film of Hg(i) oxide on the electrode which is then oxidised by PN to form HgO in a chemical step. The HgO is then reduced back to Hg(0) at the electrode potential of interest resulting in a net cathodic current. The parameters affecting the voltammetry of PN at a MFE, including the concentration of PN (10-5-10-4 M), the effect of varying scan rate (5-100 mV s-1) and pH are investigated and the kinetics of PN decay in alkaline solutions ranging from pH 9-13 are explored electrochemically.
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U2 - 10.1039/b615188d
DO - 10.1039/b615188d
M3 - Article
AN - SCOPUS:33847657524
VL - 31
SP - 394
EP - 400
JO - New Journal of Chemistry
JF - New Journal of Chemistry
SN - 1144-0546
IS - 3
ER -