The Secondary Structure of Binase in Solution Determined by 1H NMR

M. Ya Reibarkh, D. E. Nolde, E. V. Bocharov, L. I. Vasil'eva, A. A. Shulga, M. P. Kirpichnikov, A. S. Arseniev

Результат исследований: Материалы для журналаСтатьярецензирование


Nearly all resonances were assigned in the two-dimensional 1H NMR spectra of binase, guanyl-ospecific ribonuclease from Bacillus intermedius containing 109 amino acid residues. The exchange rates of amide protons with the solvent deuterium were measured in 2H2O at pH 6.7 and 30°C. Coupling constants 3J of H-NCα-H, NOE contacts, solvent exchange rates of amide protons, and indices of CαH chemical shifts were measured, and the binase secondary structure was deduced from these data. It involves three α-helices in the N-terminal part (the 6-16, 26-31, and 41-45 segments) and a β-sheet formed by five antiparallel β-strands (51-55, 71-75, 86-90, 95-99, and 104-108 segments). The binase secondary structure was compared with that of its closest homologue, barnase from B. amyloliquefaciens.

Язык оригиналаАнглийский
Страницы (с-по)794
Число страниц1
ЖурналBioorganicheskaya Khimiya
Номер выпуска10
СостояниеОпубликовано - 1997
Опубликовано для внешнего пользованияДа

ASJC Scopus subject areas

  • Medicine(all)

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