Synthesis in vivo of chimeric IgE after ballistic transfection of various mouse tissues with immunoglobulin genes

Genes for the heavy and light chains of a chimeric (mouse/human) IgE were introduced into various mouse tissues using ballistic transfection in vivo. The full-size chimeric IgE content in the blood plasma was evaluated in an immunochemical test. The IgE content in blood after its transfection of the cartilage was found to be several times lower than after transfection of the liver, spleen, or foot pad. At the same time, the content of antibodies to the xenogenous determinants of IgE was essentially independent of the tissue type. After a second transfection at the 114th day, neither chimeric IgE nor antibodies to it were found in the mouse blood. These data can be employed in selecting conditions for genetic immunization.