Studying cGMP-dependent mechanisms of vinpocetine on smooth muscle cells

I. V. Kovalev, M. B. Baskakov, A. G. Popov, A. A. Kilin, I. L. Minochenko, Yu L. Borodin, A. A. Panov, Yana Jonovna Anfinogenova, L. V. Kapilevich, M. A. Medvedev

Результат исследований: Материалы для журналаСтатья

2 Цитирования (Scopus)

Выдержка

The results of the membrane potential measurements (by the double sucrose gap junction technique) and the smooth muscle tension determination (by the mechanical force measurements) in the rat aorta showed that vinpocetine potentiates the effect of sodium nitroprusside and nitroglycerin on the smooth muscle cells. In the concentration range of 2-20 μM, vinpocetine produced a dose-dependent inhibition of the Ca2+ conductivity of the membrane and decreased the smooth muscle contractility response. At a concentration of 1 μM, the drug acted as an inhibitor of the phosphodiestherase (PDE) activity and produced the effects similar top those of dibutyryl-cGMP (rather than dibutyryl-cAMP). In the presence of 10 μM of Methylene Blue (an inhibitor of the soluble fraction of guanylate cyclase), the cGMP-dependent effects of vinpocetine were suppressed on the background of 100 μM of sodium nitroprusside, but retained on the background of 10 μM of 3-isobutyl-1-methylxanthine (IBMX), a nonspecific PDE inhibitor. IBMX acted like dibityryl-cGMP and activated the K+ conductivity of the membrane. It is suggested that cGMP-dependent effects of vinpocetine are related to its action upon the Ca2+ and Na+ (but not K+) conductivity and to the cGMP-induced increase in the contribution of sarcoplasmic calcium to the contractile response.

Язык оригиналаАнглийский
Страницы (с-по)25-28
Число страниц4
ЖурналEksperimental'naya i Klinicheskaya Farmakologiya
Том66
Номер выпуска4
СостояниеОпубликовано - 2003
Опубликовано для внешнего пользованияДа

Отпечаток

vinpocetine
Smooth Muscle Myocytes
Muscle
Cells
Nitroprusside
Membranes
Smooth Muscle
1-Methyl-3-isobutylxanthine
Muscle Tonus
Force measurement
Guanylate Cyclase
Methylene Blue
Gap Junctions
Nitroglycerin
Membrane Potentials
Sucrose
Aorta
Rats
Calcium
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Pharmacology, Toxicology and Pharmaceutics(all)

Цитировать

Kovalev, I. V., Baskakov, M. B., Popov, A. G., Kilin, A. A., Minochenko, I. L., Borodin, Y. L., ... Medvedev, M. A. (2003). Studying cGMP-dependent mechanisms of vinpocetine on smooth muscle cells. Eksperimental'naya i Klinicheskaya Farmakologiya, 66(4), 25-28.

Studying cGMP-dependent mechanisms of vinpocetine on smooth muscle cells. / Kovalev, I. V.; Baskakov, M. B.; Popov, A. G.; Kilin, A. A.; Minochenko, I. L.; Borodin, Yu L.; Panov, A. A.; Anfinogenova, Yana Jonovna; Kapilevich, L. V.; Medvedev, M. A.

В: Eksperimental'naya i Klinicheskaya Farmakologiya, Том 66, № 4, 2003, стр. 25-28.

Результат исследований: Материалы для журналаСтатья

Kovalev, IV, Baskakov, MB, Popov, AG, Kilin, AA, Minochenko, IL, Borodin, YL, Panov, AA, Anfinogenova, YJ, Kapilevich, LV & Medvedev, MA 2003, 'Studying cGMP-dependent mechanisms of vinpocetine on smooth muscle cells', Eksperimental'naya i Klinicheskaya Farmakologiya, том. 66, № 4, стр. 25-28.
Kovalev IV, Baskakov MB, Popov AG, Kilin AA, Minochenko IL, Borodin YL и соавт. Studying cGMP-dependent mechanisms of vinpocetine on smooth muscle cells. Eksperimental'naya i Klinicheskaya Farmakologiya. 2003;66(4):25-28.
Kovalev, I. V. ; Baskakov, M. B. ; Popov, A. G. ; Kilin, A. A. ; Minochenko, I. L. ; Borodin, Yu L. ; Panov, A. A. ; Anfinogenova, Yana Jonovna ; Kapilevich, L. V. ; Medvedev, M. A. / Studying cGMP-dependent mechanisms of vinpocetine on smooth muscle cells. В: Eksperimental'naya i Klinicheskaya Farmakologiya. 2003 ; Том 66, № 4. стр. 25-28.
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AU - Kovalev, I. V.

AU - Baskakov, M. B.

AU - Popov, A. G.

AU - Kilin, A. A.

AU - Minochenko, I. L.

AU - Borodin, Yu L.

AU - Panov, A. A.

AU - Anfinogenova, Yana Jonovna

AU - Kapilevich, L. V.

AU - Medvedev, M. A.

PY - 2003

Y1 - 2003

N2 - The results of the membrane potential measurements (by the double sucrose gap junction technique) and the smooth muscle tension determination (by the mechanical force measurements) in the rat aorta showed that vinpocetine potentiates the effect of sodium nitroprusside and nitroglycerin on the smooth muscle cells. In the concentration range of 2-20 μM, vinpocetine produced a dose-dependent inhibition of the Ca2+ conductivity of the membrane and decreased the smooth muscle contractility response. At a concentration of 1 μM, the drug acted as an inhibitor of the phosphodiestherase (PDE) activity and produced the effects similar top those of dibutyryl-cGMP (rather than dibutyryl-cAMP). In the presence of 10 μM of Methylene Blue (an inhibitor of the soluble fraction of guanylate cyclase), the cGMP-dependent effects of vinpocetine were suppressed on the background of 100 μM of sodium nitroprusside, but retained on the background of 10 μM of 3-isobutyl-1-methylxanthine (IBMX), a nonspecific PDE inhibitor. IBMX acted like dibityryl-cGMP and activated the K+ conductivity of the membrane. It is suggested that cGMP-dependent effects of vinpocetine are related to its action upon the Ca2+ and Na+ (but not K+) conductivity and to the cGMP-induced increase in the contribution of sarcoplasmic calcium to the contractile response.

AB - The results of the membrane potential measurements (by the double sucrose gap junction technique) and the smooth muscle tension determination (by the mechanical force measurements) in the rat aorta showed that vinpocetine potentiates the effect of sodium nitroprusside and nitroglycerin on the smooth muscle cells. In the concentration range of 2-20 μM, vinpocetine produced a dose-dependent inhibition of the Ca2+ conductivity of the membrane and decreased the smooth muscle contractility response. At a concentration of 1 μM, the drug acted as an inhibitor of the phosphodiestherase (PDE) activity and produced the effects similar top those of dibutyryl-cGMP (rather than dibutyryl-cAMP). In the presence of 10 μM of Methylene Blue (an inhibitor of the soluble fraction of guanylate cyclase), the cGMP-dependent effects of vinpocetine were suppressed on the background of 100 μM of sodium nitroprusside, but retained on the background of 10 μM of 3-isobutyl-1-methylxanthine (IBMX), a nonspecific PDE inhibitor. IBMX acted like dibityryl-cGMP and activated the K+ conductivity of the membrane. It is suggested that cGMP-dependent effects of vinpocetine are related to its action upon the Ca2+ and Na+ (but not K+) conductivity and to the cGMP-induced increase in the contribution of sarcoplasmic calcium to the contractile response.

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