Study of potential utility of new radiopharmaceuticals based on technetium-99m labeled derivative of glucose

R. Zeltchan, A. Medvedeva, I. Sinilkin, V. Chernov, E. Stasyuk, A. Rogov, E. Il'Ina, L. Larionova, V. Skuridin

Результат исследований: Материалы для книги/типы отчетовМатериалы для конференции

4 Цитирования (Scopus)

Аннотация

Purpose: to study the potential utility of 1-thio-D-glucose labeled with 99mTc for cancer imaging in laboratory animals. Materials and method: the study was carried out in cell cultures of normal CHO (Chinese hamster ovary cells CHO) and malignant tissues MCF-7 (human breast adenocarcinoma MCF-7). To evaluate the uptake of 99mTc-1-thio-D-glucose in normal and tumor tissue cells, 25 MBq of 1-thio-D-glucose labeled with 99mTc was added to the vials with 3 million cells and incubated for 30 min at room temperature. After centrifugation of the vials with cells, the supernatant was removed. The radioactivity in vials with normal and tumor cells was then measured. In addition, the study included 40 mice of C57B1/6j lines with tumor lesion of the right femur. For neoplastic lesions, Lewis lung carcinoma model was used. Following anesthesia, mice were injected intravenously with 25 MBq of 99mTc-1-thio-D-glucose. Planar scintigraphy was performed 15 minutes later in a matrix of 512x512 pixels for 5 min. Results: when measuring the radioactivity of normal and malignant cells after incubation with 99mTc-1-thio-D-glucose, it was found that the radioactivity of malignant cells was higher than that of normal cells. The mean values of radioactivity levels in normal and malignant cells were 0.3 ± 0.15 MBq and 1.07 ± 0.6 MBq, respectively. All examined animals had increased accumulation of 99mTc-1-thio-D-glucose at the tumor site. The accumulation of 99mTc-1-thio-D-glucose in the tumor was on average twice as high as compared to the symmetric region. Conclusion: The present study demonstrated that 99mTc-1-thio-D-glucose is a prospective radiopharmaceutical for cancer visualization. In addition, high accumulation of 99mTc-1-thio-D-glucose in the culture of cancer cells and in tumor tissue of animals demonstrates tumor tropism of the radiopharmaceutical.

Язык оригиналаАнглийский
Название основной публикацииPhysics of Cancer
Подзаголовок основной публикацииInterdisciplinary Problems and Clinical Applications, PC 2016: Proceedings of the International Conference on Physics of Cancer: Interdisciplinary Problems and Clinical Applications 2016
ИздательAmerican Institute of Physics Inc.
Том1760
ISBN (электронное издание)9780735414181
DOI
СостояниеОпубликовано - 2 авг 2016
СобытиеInternational Conference on Physics of Cancer: Interdisciplinary Problems and Clinical Applications 2016, PC 2016 - Tomsk, Российская Федерация
Продолжительность: 22 мар 201625 мар 2016

Конференция

КонференцияInternational Conference on Physics of Cancer: Interdisciplinary Problems and Clinical Applications 2016, PC 2016
СтранаРоссийская Федерация
ГородTomsk
Период22.3.1625.3.16

ASJC Scopus subject areas

  • Physics and Astronomy(all)

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