Structural and functional alterations in the atrioventricular node and atrioventricular ring tissue in ischaemia-induced heart failure

Joseph Yanni, Michal Maczewski, Urszula Mackiewicz, Samuel Siew, Olga Fedorenko, Andrew Atkinson, Marcus Price, Andrzej Beresewicz, Robert H. Anderson, Mark R. Boyett, Halina Dobrzynski

Результат исследований: Материалы для журналаСтатья

6 Цитирования (Scopus)

Аннотация

Heart failure (HF) causes dysfunction of the atrioventricular node (AVN) - first or second-degree heart block is a risk factor for sudden cardiac death in HF patients. The aim of the study was to determine if HF causes remodelling of the AVN and right atrioventricular ring (RAVR). HF was induced in rats (n=4) by ligation of the proximal left coronary artery, which resulted in a large infarct of the left ventricle. Sham-operated rats (n=4) were used as controls. Eight weeks after surgery, functional experiments were performed and the hearts were frozen. The body weight of HF rats was similar to control rats, but the mean heart weight of HF rats was significantly enlarged. In HF rats compared to controls, the left ventricle was dilated, left ventricular enddiastolic pressure elevated (21.0±0.6 and 5.4±0.2 mm Hg), left ventricular ejection fraction reduced (0.2±0.02 and 0.5±0.02) and left ventricular end-systolic pressure reduced (102±4.2 and 127±3.1 mm Hg). In HF rats, the in vivo and in vitro PR intervals were increased (41% and 20%), as was the Wenckebach cycle length, indicative of AVN dysfunction. The collagen content was significantly increased in the AVN and RAVR indicating fibrosis. Immunolabelling of caveolin3 (cell membrane marker) showed that there was hypertrophy in HF (cell diameter was increased by 63%, 39% in AVN, RAVR). The TUNEL assay showed that the myocytes of the AVN and RAVR in HF undergo apoptotic cell death. Immunolabelling showed that expression of HCN4 was significantly decreased in the AVN and RAVR (43% and 47%) in HF. We conclude that in HF there is remodelling of the AVN and RAVR and this remodelling may explain the AVN dysfunction.

Язык оригиналаАнглийский
Страницы (с-по)891-902
Число страниц12
ЖурналHistology and Histopathology
Том29
Номер выпуска7
СостояниеОпубликовано - 2014

ASJC Scopus subject areas

  • Histology
  • Pathology and Forensic Medicine

Fingerprint Подробные сведения о темах исследования «Structural and functional alterations in the atrioventricular node and atrioventricular ring tissue in ischaemia-induced heart failure». Вместе они формируют уникальный семантический отпечаток (fingerprint).

  • Цитировать

    Yanni, J., Maczewski, M., Mackiewicz, U., Siew, S., Fedorenko, O., Atkinson, A., Price, M., Beresewicz, A., Anderson, R. H., Boyett, M. R., & Dobrzynski, H. (2014). Structural and functional alterations in the atrioventricular node and atrioventricular ring tissue in ischaemia-induced heart failure. Histology and Histopathology, 29(7), 891-902.