Specific visualization of tumor cells using upconversion nanophosphors

E. A. Grebenik, A. N. Generalova, A. V. Nechaev, E. V. Khaydukov, K. E. Mironova, O. A. Stremovskiy, E. N. Lebedenko, A. V. Zvyagin, S. M. Deyev

Результат исследований: Материалы для журналаСтатья

5 Цитирования (Scopus)

Аннотация

The development of targeted constructs on the basis of photoluminescent nanoparticles with a high photo- and chemical stability and absorption/emission spectra in the "transparency window" of biological tissues is an important focus area of present-day medical diagnostics. In this work, a targeted two-component construct on the basis of upconversion nanophosphors (UCNPs) and anti-tumor 4D5 scFv was developed for selective labeling of tumor cells overexpressing the HER2 tumor marker characteristic of a number of human malignant tumors. A high affinity barnase: barstar (Bn: Bs) protein pair, which exhibits high stability in a wide range of pH and temperatures, was exploited as a molecular adapter providing self-assembly of the two-component construct. High selectivity for the binding of the two-component 4D5 scFv-Bn: UCNP-Bs construct to human breast adenocarcinoma SK-BR-3 cells overexpressing HER2 was demonstrated. This approach provides an opportunity to produce similar constructs for the visualization of different specific markers in pathogenic tissues, including malignant tumors.

Язык оригиналаАнглийский
Страницы (с-по)48-53
Число страниц6
ЖурналActa Naturae
Том6
Номер выпуска23
СостояниеОпубликовано - 2014
Опубликовано для внешнего пользованияДа

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Medicine
  • Molecular Biology

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  • Цитировать

    Grebenik, E. A., Generalova, A. N., Nechaev, A. V., Khaydukov, E. V., Mironova, K. E., Stremovskiy, O. A., Lebedenko, E. N., Zvyagin, A. V., & Deyev, S. M. (2014). Specific visualization of tumor cells using upconversion nanophosphors. Acta Naturae, 6(23), 48-53.