“Solvent/non-solvent” treatment as a method for non-covalent immobilization of gelatin on the surface of poly(L-lactic acid) electrospun scaffolds

S. I. Goreninskii, R. O. Guliaev, K. S. Stankevich, N. V. Danilenko, E. N. Bolbasov, A. S. Golovkin, A. I. Mishanin, V. D. Filimonov, S. I. Tverdokhlebov

Результат исследований: Материалы для журналаСтатья

Выдержка

In the present study, we report a simple and efficient method of gelatin immobilization on the surface of PLA electrospun fibers using pre-treatment with a mixture of toluene and ethanol allowing to form swelled surface layer followed by gelatin adsorption from its solution in PBS. Our results demonstrate that gelatin immobilization leads to a decrease in the water contact angle from 120° to 0° enhances scaffold strength up to 50%, and doubles the number of adhered cells and their average area. We observed that the maximum amount of gelatin (0.07 ± 0.01 mg per cm3 of the scaffold) was immobilized during the first five minutes of exposure to the gelatin solution. Modified scaffolds demonstrated increased strength.

Язык оригиналаАнглийский
Страницы (с-по)137-140
Число страниц4
ЖурналColloids and Surfaces B: Biointerfaces
Том177
DOI
СостояниеОпубликовано - 1 мая 2019

Отпечаток

lactic acid
gelatins
Gelatin
Lactic acid
immobilization
Scaffolds
Immobilization
Contact angle
Toluene
Ethanol
Adsorption
Fibers
pretreatment
toluene
surface layers
ethyl alcohol
Cell Count
poly(lactic acid)
Water
adsorption

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Цитировать

“Solvent/non-solvent” treatment as a method for non-covalent immobilization of gelatin on the surface of poly(L-lactic acid) electrospun scaffolds. / Goreninskii, S. I.; Guliaev, R. O.; Stankevich, K. S.; Danilenko, N. V.; Bolbasov, E. N.; Golovkin, A. S.; Mishanin, A. I.; Filimonov, V. D.; Tverdokhlebov, S. I.

В: Colloids and Surfaces B: Biointerfaces, Том 177, 01.05.2019, стр. 137-140.

Результат исследований: Материалы для журналаСтатья

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abstract = "In the present study, we report a simple and efficient method of gelatin immobilization on the surface of PLA electrospun fibers using pre-treatment with a mixture of toluene and ethanol allowing to form swelled surface layer followed by gelatin adsorption from its solution in PBS. Our results demonstrate that gelatin immobilization leads to a decrease in the water contact angle from 120° to 0° enhances scaffold strength up to 50{\%}, and doubles the number of adhered cells and their average area. We observed that the maximum amount of gelatin (0.07 ± 0.01 mg per cm3 of the scaffold) was immobilized during the first five minutes of exposure to the gelatin solution. Modified scaffolds demonstrated increased strength.",
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author = "Goreninskii, {S. I.} and Guliaev, {R. O.} and Stankevich, {K. S.} and Danilenko, {N. V.} and Bolbasov, {E. N.} and Golovkin, {A. S.} and Mishanin, {A. I.} and Filimonov, {V. D.} and Tverdokhlebov, {S. I.}",
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T1 - “Solvent/non-solvent” treatment as a method for non-covalent immobilization of gelatin on the surface of poly(L-lactic acid) electrospun scaffolds

AU - Goreninskii, S. I.

AU - Guliaev, R. O.

AU - Stankevich, K. S.

AU - Danilenko, N. V.

AU - Bolbasov, E. N.

AU - Golovkin, A. S.

AU - Mishanin, A. I.

AU - Filimonov, V. D.

AU - Tverdokhlebov, S. I.

PY - 2019/5/1

Y1 - 2019/5/1

N2 - In the present study, we report a simple and efficient method of gelatin immobilization on the surface of PLA electrospun fibers using pre-treatment with a mixture of toluene and ethanol allowing to form swelled surface layer followed by gelatin adsorption from its solution in PBS. Our results demonstrate that gelatin immobilization leads to a decrease in the water contact angle from 120° to 0° enhances scaffold strength up to 50%, and doubles the number of adhered cells and their average area. We observed that the maximum amount of gelatin (0.07 ± 0.01 mg per cm3 of the scaffold) was immobilized during the first five minutes of exposure to the gelatin solution. Modified scaffolds demonstrated increased strength.

AB - In the present study, we report a simple and efficient method of gelatin immobilization on the surface of PLA electrospun fibers using pre-treatment with a mixture of toluene and ethanol allowing to form swelled surface layer followed by gelatin adsorption from its solution in PBS. Our results demonstrate that gelatin immobilization leads to a decrease in the water contact angle from 120° to 0° enhances scaffold strength up to 50%, and doubles the number of adhered cells and their average area. We observed that the maximum amount of gelatin (0.07 ± 0.01 mg per cm3 of the scaffold) was immobilized during the first five minutes of exposure to the gelatin solution. Modified scaffolds demonstrated increased strength.

KW - Electrospun scaffolds

KW - Gelatin

KW - Polylactic acid

KW - Surface modification

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