Silver nanoparticles enhance survival of white spot syndrome virus infected Penaeus vannamei shrimps by activation of its immunological system

Alba R. Ochoa-Meza, Ana R. Álvarez-Sánchez, Carlos R. Romo-Quiñonez, Aarón Barraza, Francisco J. Magallón-Barajas, Alexis Chávez-Sánchez, Juan Carlos García-Ramos, Yanis Toledano-Magaña, Nina Bogdanchikova, Alexey Pestryakov, Claudio Humberto Mejía-Ruiz

Результат исследований: Материалы для журналаСтатья

Выдержка

The global aquaculture has shown an impressive growth in the last decades contributing with a major part of total food fish supply. However, it also helps in the spread of diseases that in turn, causes great economic losses. The White Spot Syndrome Virus (WSSV) is one of the major viral pathogen for the shrimp aquaculture industry. Several attempts to eliminate the virus in the shrimp have been addressed without achieving a long-term effectiveness. In this work, we determine the capacity of the commercial non-toxic PVP-coated silver nanoparticles to promote the response of the immune system of WSSV-infected shrimps with or without an excess of iron ions. Our results showed that a single dose of metallic silver in the nanomolar range (111 nmol/shrimp), which is equivalent to 12 ng/mL of silver nanoparticles, produces 20% survival of treated infected shrimps. The same concentration administered in healthy shrimps do not show histological evidence of damage. The observed survival rate could be associated with the increase of almost 2-fold of LGBP expression levels compared with non-treated infected shrimps. LGBP is a key gene of shrimp immunological response and its up-regulation is most probably induced by the recognition of silver nanoparticles coating by specific pathogen-associated molecular pattern recognition proteins (PAMPs) of shrimp. Increased LGBP expression levels was observed even with a 10-fold lower dose of silver nanoparticles (1.2 ng/shrimp, 0.011 nmol of metallic silver/shrimp). The increase in LGBP expression levels was also observed even in the presence of iron ion excess, a condition that favors virus proliferation. Those results showed that a single dose of a slight amount of silver nanoparticles were capable to enhance the response of shrimp immune system without toxic effects in healthy shrimps. This response could be enhanced by administration of other doses and might represent an important alternative for the treatment of a disease that has still no cure, white spot syndrome virus.

Язык оригиналаАнглийский
Страницы (с-по)1083-1089
Число страниц7
ЖурналFish and Shellfish Immunology
Том84
DOI
СостояниеОпубликовано - 1 янв 2019

Отпечаток

nanosilver
White spot syndrome virus
Litopenaeus vannamei
Viruses
Silver
silver
virus
shrimp
Chemical activation
Nanoparticles
Aquaculture
Immune system
immune system
Iron
pathogen
Ions
fold
iron
aquaculture industry
dosage

ASJC Scopus subject areas

  • Environmental Chemistry
  • Aquatic Science

Цитировать

Ochoa-Meza, A. R., Álvarez-Sánchez, A. R., Romo-Quiñonez, C. R., Barraza, A., Magallón-Barajas, F. J., Chávez-Sánchez, A., ... Mejía-Ruiz, C. H. (2019). Silver nanoparticles enhance survival of white spot syndrome virus infected Penaeus vannamei shrimps by activation of its immunological system. Fish and Shellfish Immunology, 84, 1083-1089. https://doi.org/10.1016/j.fsi.2018.10.007

Silver nanoparticles enhance survival of white spot syndrome virus infected Penaeus vannamei shrimps by activation of its immunological system. / Ochoa-Meza, Alba R.; Álvarez-Sánchez, Ana R.; Romo-Quiñonez, Carlos R.; Barraza, Aarón; Magallón-Barajas, Francisco J.; Chávez-Sánchez, Alexis; García-Ramos, Juan Carlos; Toledano-Magaña, Yanis; Bogdanchikova, Nina; Pestryakov, Alexey; Mejía-Ruiz, Claudio Humberto.

В: Fish and Shellfish Immunology, Том 84, 01.01.2019, стр. 1083-1089.

Результат исследований: Материалы для журналаСтатья

Ochoa-Meza, AR, Álvarez-Sánchez, AR, Romo-Quiñonez, CR, Barraza, A, Magallón-Barajas, FJ, Chávez-Sánchez, A, García-Ramos, JC, Toledano-Magaña, Y, Bogdanchikova, N, Pestryakov, A & Mejía-Ruiz, CH 2019, 'Silver nanoparticles enhance survival of white spot syndrome virus infected Penaeus vannamei shrimps by activation of its immunological system', Fish and Shellfish Immunology, том. 84, стр. 1083-1089. https://doi.org/10.1016/j.fsi.2018.10.007
Ochoa-Meza AR, Álvarez-Sánchez AR, Romo-Quiñonez CR, Barraza A, Magallón-Barajas FJ, Chávez-Sánchez A и соавт. Silver nanoparticles enhance survival of white spot syndrome virus infected Penaeus vannamei shrimps by activation of its immunological system. Fish and Shellfish Immunology. 2019 Янв. 1;84:1083-1089. https://doi.org/10.1016/j.fsi.2018.10.007
Ochoa-Meza, Alba R. ; Álvarez-Sánchez, Ana R. ; Romo-Quiñonez, Carlos R. ; Barraza, Aarón ; Magallón-Barajas, Francisco J. ; Chávez-Sánchez, Alexis ; García-Ramos, Juan Carlos ; Toledano-Magaña, Yanis ; Bogdanchikova, Nina ; Pestryakov, Alexey ; Mejía-Ruiz, Claudio Humberto. / Silver nanoparticles enhance survival of white spot syndrome virus infected Penaeus vannamei shrimps by activation of its immunological system. В: Fish and Shellfish Immunology. 2019 ; Том 84. стр. 1083-1089.
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T1 - Silver nanoparticles enhance survival of white spot syndrome virus infected Penaeus vannamei shrimps by activation of its immunological system

AU - Ochoa-Meza, Alba R.

AU - Álvarez-Sánchez, Ana R.

AU - Romo-Quiñonez, Carlos R.

AU - Barraza, Aarón

AU - Magallón-Barajas, Francisco J.

AU - Chávez-Sánchez, Alexis

AU - García-Ramos, Juan Carlos

AU - Toledano-Magaña, Yanis

AU - Bogdanchikova, Nina

AU - Pestryakov, Alexey

AU - Mejía-Ruiz, Claudio Humberto

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