S-Nitrosothiol measurements in biological systems

Andrew Gow, Allan Doctor, Joan Mannick, Benjamin Gaston

Результат исследований: Материалы для журналаОбзорная статья

84 Цитирования (Scopus)

Выдержка

S-Nitrosothiol (SNO) cysteine modifications are regulated signaling reactions that dramatically affect, and are affected by, protein conformation. The lability of the S{single bond}NO bond can make SNO-modified proteins cumbersome to measure accurately. Here, we review methodologies for detecting SNO modifications in biology. There are three caveats. (1) Many assays for biological SNOs are used near the limit of detection: standard curves must be in the biologically relevant concentration range. (2) The assays that are most reliable are those that modify SNO protein or peptide chemistry the least. (3) Each result should be quantitatively validated using more than one assay. Improved assays are needed and are in development.

Язык оригиналаАнглийский
Страницы (с-по)140-151
Число страниц12
ЖурналJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Том851
Номер выпуска1-2
DOI
СостояниеОпубликовано - 15 мая 2007
Опубликовано для внешнего пользованияДа

Отпечаток

S-Nitrosothiols
Biological systems
Assays
Protein Conformation
Proteins
Biological Assay
Cysteine
Conformations
Limit of Detection
Peptides

ASJC Scopus subject areas

  • Biochemistry

Цитировать

S-Nitrosothiol measurements in biological systems. / Gow, Andrew; Doctor, Allan; Mannick, Joan; Gaston, Benjamin.

В: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Том 851, № 1-2, 15.05.2007, стр. 140-151.

Результат исследований: Материалы для журналаОбзорная статья

Gow, Andrew ; Doctor, Allan ; Mannick, Joan ; Gaston, Benjamin. / S-Nitrosothiol measurements in biological systems. В: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2007 ; Том 851, № 1-2. стр. 140-151.
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