Role of spleen-derived macrophages in ozone-induced lung inflammation and injury

Mary Francis, Richard Sun, Jessica A. Cervelli, Hyejeong Choi, Mili Mandal, Elena V. Abramova, Andrew J. Gow, Jeffrey D. Laskin, Debra L. Laskin

Результат исследований: Материалы для журналаСтатья

8 Цитирования (Scopus)

Выдержка

Macrophages and inflammatory mediators have been implicated in ozone toxicity. In these studies, we used splenectomized (SPX) mice to assess the contribution of splenic monocytes to pulmonary inflammation and injury induced by ozone. Cells and tissue were collected 24-72h after exposure of mice to air or ozone (0.8 ppm, 3 h). Following ozone exposure, increased numbers of pro-inflammatory CD11b +Ly6CHi and anti-inflammatory CD11b + Ly6CLo monocytes were observed in spleens of control (CTL) mice. CD11b + Ly6CHi and MMP-9+pro-inflammatory macrophages were also observed in lungs of CTL mice after ozone, along with CD11b+ Ly6CLo and mannose receptor (MR)+ anti-inflammatory macrophages. This was accompanied by increased lung expression of proteins involved in monocyte/macrophage trafficking including CCL3, CCL4, CCR1, and AT1R. Splenectomy resulted in decreases in pro-inflammatory macrophages in the lung and down regulation of CCR2, CCL2, and CCL4, but increases in CD11b+ Ly6CLo anti-inflammatory macrophages. CD11b+Ly6G+Ly6C+ granulocytic (G)- and monocytic (M)-myeloid derived suppressor cells (MDSC)s were also detected in the lungs and spleens of CTL mice; these increased after ozone exposure. Splenectomy was associated with a decrease in G-MDSCs in the lung, with no effect on M-MDSCs. Changes in lung macrophage subpopulations and MDSCs in SPX mice were correlated with reduced ozone toxicity, as measured by decreases in bronchoalveolar lavage protein content and reduced 4- hydroxynonenal expression in the lung. These data suggest that the spleen is a source of pro-inflammatory/cytotoxic macrophages that contribute to ozone-induced lung injury, inflammation, and oxidative stress.

Язык оригиналаАнглийский
Страницы (с-по)182-195
Число страниц14
ЖурналToxicological Sciences
Том155
Номер выпуска1
DOI
СостояниеОпубликовано - 2017
Опубликовано для внешнего пользованияДа

Отпечаток

Macrophages
Ozone
Lung Injury
Pneumonia
Spleen
Lung
Monocytes
Anti-Inflammatory Agents
Splenectomy
Toxicity
Oxidative stress
Bronchoalveolar Lavage
Matrix Metalloproteinases
Proteins
Oxidative Stress
Down-Regulation
Air
Tissue
Myeloid-Derived Suppressor Cells

ASJC Scopus subject areas

  • Toxicology

Цитировать

Francis, M., Sun, R., Cervelli, J. A., Choi, H., Mandal, M., Abramova, E. V., ... Laskin, D. L. (2017). Role of spleen-derived macrophages in ozone-induced lung inflammation and injury. Toxicological Sciences, 155(1), 182-195. https://doi.org/10.1093/toxsci/kfw192

Role of spleen-derived macrophages in ozone-induced lung inflammation and injury. / Francis, Mary; Sun, Richard; Cervelli, Jessica A.; Choi, Hyejeong; Mandal, Mili; Abramova, Elena V.; Gow, Andrew J.; Laskin, Jeffrey D.; Laskin, Debra L.

В: Toxicological Sciences, Том 155, № 1, 2017, стр. 182-195.

Результат исследований: Материалы для журналаСтатья

Francis, M, Sun, R, Cervelli, JA, Choi, H, Mandal, M, Abramova, EV, Gow, AJ, Laskin, JD & Laskin, DL 2017, 'Role of spleen-derived macrophages in ozone-induced lung inflammation and injury', Toxicological Sciences, том. 155, № 1, стр. 182-195. https://doi.org/10.1093/toxsci/kfw192
Francis M, Sun R, Cervelli JA, Choi H, Mandal M, Abramova EV и соавт. Role of spleen-derived macrophages in ozone-induced lung inflammation and injury. Toxicological Sciences. 2017;155(1):182-195. https://doi.org/10.1093/toxsci/kfw192
Francis, Mary ; Sun, Richard ; Cervelli, Jessica A. ; Choi, Hyejeong ; Mandal, Mili ; Abramova, Elena V. ; Gow, Andrew J. ; Laskin, Jeffrey D. ; Laskin, Debra L. / Role of spleen-derived macrophages in ozone-induced lung inflammation and injury. В: Toxicological Sciences. 2017 ; Том 155, № 1. стр. 182-195.
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abstract = "Macrophages and inflammatory mediators have been implicated in ozone toxicity. In these studies, we used splenectomized (SPX) mice to assess the contribution of splenic monocytes to pulmonary inflammation and injury induced by ozone. Cells and tissue were collected 24-72h after exposure of mice to air or ozone (0.8 ppm, 3 h). Following ozone exposure, increased numbers of pro-inflammatory CD11b +Ly6CHi and anti-inflammatory CD11b + Ly6CLo monocytes were observed in spleens of control (CTL) mice. CD11b + Ly6CHi and MMP-9+pro-inflammatory macrophages were also observed in lungs of CTL mice after ozone, along with CD11b+ Ly6CLo and mannose receptor (MR)+ anti-inflammatory macrophages. This was accompanied by increased lung expression of proteins involved in monocyte/macrophage trafficking including CCL3, CCL4, CCR1, and AT1R. Splenectomy resulted in decreases in pro-inflammatory macrophages in the lung and down regulation of CCR2, CCL2, and CCL4, but increases in CD11b+ Ly6CLo anti-inflammatory macrophages. CD11b+Ly6G+Ly6C+ granulocytic (G)- and monocytic (M)-myeloid derived suppressor cells (MDSC)s were also detected in the lungs and spleens of CTL mice; these increased after ozone exposure. Splenectomy was associated with a decrease in G-MDSCs in the lung, with no effect on M-MDSCs. Changes in lung macrophage subpopulations and MDSCs in SPX mice were correlated with reduced ozone toxicity, as measured by decreases in bronchoalveolar lavage protein content and reduced 4- hydroxynonenal expression in the lung. These data suggest that the spleen is a source of pro-inflammatory/cytotoxic macrophages that contribute to ozone-induced lung injury, inflammation, and oxidative stress.",
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T1 - Role of spleen-derived macrophages in ozone-induced lung inflammation and injury

AU - Francis, Mary

AU - Sun, Richard

AU - Cervelli, Jessica A.

AU - Choi, Hyejeong

AU - Mandal, Mili

AU - Abramova, Elena V.

AU - Gow, Andrew J.

AU - Laskin, Jeffrey D.

AU - Laskin, Debra L.

PY - 2017

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N2 - Macrophages and inflammatory mediators have been implicated in ozone toxicity. In these studies, we used splenectomized (SPX) mice to assess the contribution of splenic monocytes to pulmonary inflammation and injury induced by ozone. Cells and tissue were collected 24-72h after exposure of mice to air or ozone (0.8 ppm, 3 h). Following ozone exposure, increased numbers of pro-inflammatory CD11b +Ly6CHi and anti-inflammatory CD11b + Ly6CLo monocytes were observed in spleens of control (CTL) mice. CD11b + Ly6CHi and MMP-9+pro-inflammatory macrophages were also observed in lungs of CTL mice after ozone, along with CD11b+ Ly6CLo and mannose receptor (MR)+ anti-inflammatory macrophages. This was accompanied by increased lung expression of proteins involved in monocyte/macrophage trafficking including CCL3, CCL4, CCR1, and AT1R. Splenectomy resulted in decreases in pro-inflammatory macrophages in the lung and down regulation of CCR2, CCL2, and CCL4, but increases in CD11b+ Ly6CLo anti-inflammatory macrophages. CD11b+Ly6G+Ly6C+ granulocytic (G)- and monocytic (M)-myeloid derived suppressor cells (MDSC)s were also detected in the lungs and spleens of CTL mice; these increased after ozone exposure. Splenectomy was associated with a decrease in G-MDSCs in the lung, with no effect on M-MDSCs. Changes in lung macrophage subpopulations and MDSCs in SPX mice were correlated with reduced ozone toxicity, as measured by decreases in bronchoalveolar lavage protein content and reduced 4- hydroxynonenal expression in the lung. These data suggest that the spleen is a source of pro-inflammatory/cytotoxic macrophages that contribute to ozone-induced lung injury, inflammation, and oxidative stress.

AB - Macrophages and inflammatory mediators have been implicated in ozone toxicity. In these studies, we used splenectomized (SPX) mice to assess the contribution of splenic monocytes to pulmonary inflammation and injury induced by ozone. Cells and tissue were collected 24-72h after exposure of mice to air or ozone (0.8 ppm, 3 h). Following ozone exposure, increased numbers of pro-inflammatory CD11b +Ly6CHi and anti-inflammatory CD11b + Ly6CLo monocytes were observed in spleens of control (CTL) mice. CD11b + Ly6CHi and MMP-9+pro-inflammatory macrophages were also observed in lungs of CTL mice after ozone, along with CD11b+ Ly6CLo and mannose receptor (MR)+ anti-inflammatory macrophages. This was accompanied by increased lung expression of proteins involved in monocyte/macrophage trafficking including CCL3, CCL4, CCR1, and AT1R. Splenectomy resulted in decreases in pro-inflammatory macrophages in the lung and down regulation of CCR2, CCL2, and CCL4, but increases in CD11b+ Ly6CLo anti-inflammatory macrophages. CD11b+Ly6G+Ly6C+ granulocytic (G)- and monocytic (M)-myeloid derived suppressor cells (MDSC)s were also detected in the lungs and spleens of CTL mice; these increased after ozone exposure. Splenectomy was associated with a decrease in G-MDSCs in the lung, with no effect on M-MDSCs. Changes in lung macrophage subpopulations and MDSCs in SPX mice were correlated with reduced ozone toxicity, as measured by decreases in bronchoalveolar lavage protein content and reduced 4- hydroxynonenal expression in the lung. These data suggest that the spleen is a source of pro-inflammatory/cytotoxic macrophages that contribute to ozone-induced lung injury, inflammation, and oxidative stress.

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KW - Macrophages

KW - Myeloid derived suppressor cells

KW - Ozone

KW - Spleen

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