Regulatory influence of opiod peptides on the activity of antioxidant enzymes and prostanoid system in myocardium during stress

T. Yu Rebrova, L. N. Maslov, Yu B. Lishmanov

Результат исследований: Материалы для журналаСтатья

Выдержка

The six hour stress in rats was modeled by the method of O. Desiderato. Cardiac damage was estimated by myocardial uptace of radioactive 99Tc-pyrpphosphate. Intravenous administration of mixed mu and delta opioid receptor agonist, D- Ala2,Leu5,Arg 6-enkephalin (dalargin), (non-penetrating through the blood brain barrier) at a dose of 0.1 mg/kg before stress decreased stress-induced 99Tc-pyroposphate uptake. Pretreatment with dalargin completely abolished the stress-induced increase in conjugated dienes and malondialdehyde levels in myocardium and prevented a decrease in total lipid soluble antioxidant levels in the heart after stress exposure. Stress is accompanied by an increase in activity of catalase, glutathione peroxidase, glutathione reductase and a decrease in activity of superoxide dismutase (SOD). Pretreatment with dalargin completely reversed stress-induced decrease in SOD activity but had minor effect on the activity of other antioxidant enzymes. Stress also resulted in an increase in myocardial content of thromboxane and a decrease in prostacycline and prostaglandin E levels in the heart. Pretreatment with dalargin completely eliminated these stress-induced changes in myocardial prostanoid levels. We propose that stress-induced heart injury depends on the activation of lipid peroxidation processes and changes in the prostanoid levels in the heart. Cardioprotective effect of dalargin during stress may be mediated via peripheral mu and delta opioid receptor stimulation and an inhibition of lipid peroxidation processes through SOD activation and also a recovery of normal prostanoid levels in the heart.

Язык оригиналаАнглийский
Страницы (с-по)177-184
Число страниц8
ЖурналBiomeditsinskaya Khimiya
Том51
Номер выпуска2
СостояниеОпубликовано - 2005

Отпечаток

Enkephalins
Prostaglandins
Myocardium
Antioxidants
Peptides
Superoxide Dismutase
Enzymes
delta Opioid Receptor
mu Opioid Receptor
Lipid Peroxidation
Heart Injuries
Glutathione Reductase
Thromboxanes
Glutathione Peroxidase
Prostaglandins E
Malondialdehyde
Blood-Brain Barrier
Intravenous Administration
Catalase
Lipids

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry
  • Molecular Biology
  • Biotechnology
  • Pharmacology

Цитировать

Regulatory influence of opiod peptides on the activity of antioxidant enzymes and prostanoid system in myocardium during stress. / Rebrova, T. Yu; Maslov, L. N.; Lishmanov, Yu B.

В: Biomeditsinskaya Khimiya, Том 51, № 2, 2005, стр. 177-184.

Результат исследований: Материалы для журналаСтатья

@article{ea00ccb80e50491fbbae539ee066f49e,
title = "Regulatory influence of opiod peptides on the activity of antioxidant enzymes and prostanoid system in myocardium during stress",
abstract = "The six hour stress in rats was modeled by the method of O. Desiderato. Cardiac damage was estimated by myocardial uptace of radioactive 99Tc-pyrpphosphate. Intravenous administration of mixed mu and delta opioid receptor agonist, D- Ala2,Leu5,Arg 6-enkephalin (dalargin), (non-penetrating through the blood brain barrier) at a dose of 0.1 mg/kg before stress decreased stress-induced 99Tc-pyroposphate uptake. Pretreatment with dalargin completely abolished the stress-induced increase in conjugated dienes and malondialdehyde levels in myocardium and prevented a decrease in total lipid soluble antioxidant levels in the heart after stress exposure. Stress is accompanied by an increase in activity of catalase, glutathione peroxidase, glutathione reductase and a decrease in activity of superoxide dismutase (SOD). Pretreatment with dalargin completely reversed stress-induced decrease in SOD activity but had minor effect on the activity of other antioxidant enzymes. Stress also resulted in an increase in myocardial content of thromboxane and a decrease in prostacycline and prostaglandin E levels in the heart. Pretreatment with dalargin completely eliminated these stress-induced changes in myocardial prostanoid levels. We propose that stress-induced heart injury depends on the activation of lipid peroxidation processes and changes in the prostanoid levels in the heart. Cardioprotective effect of dalargin during stress may be mediated via peripheral mu and delta opioid receptor stimulation and an inhibition of lipid peroxidation processes through SOD activation and also a recovery of normal prostanoid levels in the heart.",
keywords = "Antioxidants, Heart damage, Lipid peroxidation, Opioid receptors, Prostacycline, Stress, Thromboxane",
author = "Rebrova, {T. Yu} and Maslov, {L. N.} and Lishmanov, {Yu B.}",
year = "2005",
language = "English",
volume = "51",
pages = "177--184",
journal = "Biomeditsinskaya Khimiya",
issn = "2310-6905",
publisher = "Rossiiskaya Akademiya Meditsinskikh Nauk",
number = "2",

}

TY - JOUR

T1 - Regulatory influence of opiod peptides on the activity of antioxidant enzymes and prostanoid system in myocardium during stress

AU - Rebrova, T. Yu

AU - Maslov, L. N.

AU - Lishmanov, Yu B.

PY - 2005

Y1 - 2005

N2 - The six hour stress in rats was modeled by the method of O. Desiderato. Cardiac damage was estimated by myocardial uptace of radioactive 99Tc-pyrpphosphate. Intravenous administration of mixed mu and delta opioid receptor agonist, D- Ala2,Leu5,Arg 6-enkephalin (dalargin), (non-penetrating through the blood brain barrier) at a dose of 0.1 mg/kg before stress decreased stress-induced 99Tc-pyroposphate uptake. Pretreatment with dalargin completely abolished the stress-induced increase in conjugated dienes and malondialdehyde levels in myocardium and prevented a decrease in total lipid soluble antioxidant levels in the heart after stress exposure. Stress is accompanied by an increase in activity of catalase, glutathione peroxidase, glutathione reductase and a decrease in activity of superoxide dismutase (SOD). Pretreatment with dalargin completely reversed stress-induced decrease in SOD activity but had minor effect on the activity of other antioxidant enzymes. Stress also resulted in an increase in myocardial content of thromboxane and a decrease in prostacycline and prostaglandin E levels in the heart. Pretreatment with dalargin completely eliminated these stress-induced changes in myocardial prostanoid levels. We propose that stress-induced heart injury depends on the activation of lipid peroxidation processes and changes in the prostanoid levels in the heart. Cardioprotective effect of dalargin during stress may be mediated via peripheral mu and delta opioid receptor stimulation and an inhibition of lipid peroxidation processes through SOD activation and also a recovery of normal prostanoid levels in the heart.

AB - The six hour stress in rats was modeled by the method of O. Desiderato. Cardiac damage was estimated by myocardial uptace of radioactive 99Tc-pyrpphosphate. Intravenous administration of mixed mu and delta opioid receptor agonist, D- Ala2,Leu5,Arg 6-enkephalin (dalargin), (non-penetrating through the blood brain barrier) at a dose of 0.1 mg/kg before stress decreased stress-induced 99Tc-pyroposphate uptake. Pretreatment with dalargin completely abolished the stress-induced increase in conjugated dienes and malondialdehyde levels in myocardium and prevented a decrease in total lipid soluble antioxidant levels in the heart after stress exposure. Stress is accompanied by an increase in activity of catalase, glutathione peroxidase, glutathione reductase and a decrease in activity of superoxide dismutase (SOD). Pretreatment with dalargin completely reversed stress-induced decrease in SOD activity but had minor effect on the activity of other antioxidant enzymes. Stress also resulted in an increase in myocardial content of thromboxane and a decrease in prostacycline and prostaglandin E levels in the heart. Pretreatment with dalargin completely eliminated these stress-induced changes in myocardial prostanoid levels. We propose that stress-induced heart injury depends on the activation of lipid peroxidation processes and changes in the prostanoid levels in the heart. Cardioprotective effect of dalargin during stress may be mediated via peripheral mu and delta opioid receptor stimulation and an inhibition of lipid peroxidation processes through SOD activation and also a recovery of normal prostanoid levels in the heart.

KW - Antioxidants

KW - Heart damage

KW - Lipid peroxidation

KW - Opioid receptors

KW - Prostacycline

KW - Stress

KW - Thromboxane

UR - http://www.scopus.com/inward/record.url?scp=20144386294&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=20144386294&partnerID=8YFLogxK

M3 - Article

C2 - 15945351

AN - SCOPUS:20144386294

VL - 51

SP - 177

EP - 184

JO - Biomeditsinskaya Khimiya

JF - Biomeditsinskaya Khimiya

SN - 2310-6905

IS - 2

ER -