Recombinant targeted toxin based on HER2-specific DARPin possesses a strong selective cytotoxic effect in vitro and a potent antitumor activity in vivo

Evgeniya Sokolova, Galina Proshkina, Olga Kutova, Olga Shilova, Anastasiya Ryabova, Alexey Schulga, Oleg Stremovskiy, Tatiana Zdobnova, Irina Balalaeva, Sergey Deyev

Результат исследований: Материалы для журналаСтатья

27 Цитирования (Scopus)

Аннотация

DARPins fused with other proteins are promising non-immunoglobulin scaffolds for specific binding to target cells. In this study HER2-specific DARPin (DARPin-9-29) was used as a tumor-targeting moiety for the delivery of a cytotoxic agent - the fragment of Pseudomonas aeruginosa exotoxin A. It was determined that DARPin-PE40 possesses a considerable cytotoxic activity and induces apoptosis in HER2-positive cells. Cytotoxic effect of DARPin-PE40 strongly correlates with the HER2 expression level. The effect of intravenous administration of DARPin-PE40 was tested in the xenograft model of breast cancer. It was shown that treatment of animals with DARPin-PE40 caused strong and prolonged suppression of xenograft tumor growth.

Язык оригиналаАнглийский
Страницы (с-по)48-56
Число страниц9
ЖурналJournal of Controlled Release
Том233
DOI
СостояниеОпубликовано - 10 июл 2016
Опубликовано для внешнего пользованияДа

ASJC Scopus subject areas

  • Pharmaceutical Science

Fingerprint Подробные сведения о темах исследования «Recombinant targeted toxin based on HER2-specific DARPin possesses a strong selective cytotoxic effect in vitro and a potent antitumor activity in vivo». Вместе они формируют уникальный семантический отпечаток (fingerprint).

  • Цитировать