Recombinant barnase as a label in ELISA

Sergey A. Yazynin, Sergey M. Deyev

Результат исследований: Материалы для журналаСтатья

5 Цитирования (Scopus)

Аннотация

Recombinant barnase was proposed as a label in the enzyme-linked immunosorbent assay (ELISA). Barnase-conjugated pig transferrin was prepared by the periodate oxidation procedure. Solid-phase bound barnase activity was determined from the change in RNA-ethidium bromide complex fluorescence upon RNA hydrolysis. The sensitivity of transferrin-barnase conjugate determination in ELISA was no less than 5 ng per well. The conjugate was applied in competition ELISA for free transferrin determination.

Язык оригиналаАнглийский
Страницы (с-по)99-102
Число страниц4
ЖурналFEBS Letters
Том388
Номер выпуска2-3
DOI
СостояниеОпубликовано - 17 июн 1996
Опубликовано для внешнего пользованияДа

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ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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