Radiolabeled Complex of Antimicrobial Peptides UBI29-41 and UBI18-35 Labeled with 99mTc for Differential Diagnosis of Bone Infection of the Limbs

V. D. Zavadovskaya, M. S. Larkina, E. S. Stasyuk, M. A. Zorkaltsev, V. D. Udodov, V. V. Ivanov, N. M. Shevtsova, A. S. Rogov, A. Ya Lushchyk, A. V. Yantsevich, M. V. Belousov, A. N. Baikov, V. S. Skuridin, A. G. Pershina

Результат исследований: Материалы для журналаСтатьярецензирование

Аннотация

We studied an original radiolabeled complex of antimicrobial peptides UBI29-41 and UBI18-35, ubiquicidin derivatives, for distinguishing between bacterial and aseptic inflammation. For radiolabeling of the peptides with technetium-99m, a bifunctional chelating agent succinimide-1-yl 6-(bis(pyridin-2-ylmethyl)amino)hexanoate was used. The obtained complexes 99mТс-DPAH-UBI29-41 and 99mТс-DPAH-UBI18-35 had radiolabeling yield >80% and radiochemical purity >96%. Accumulation of the complexes in the focus of bacterial inflammation in bone structures and the absence of this complex in the site of aseptic inflammation was confirmed in a rat model of traumatic osteomyelitis by single-photon emission computed tomography.

Язык оригиналаАнглийский
Страницы (с-по)415-419
Число страниц5
ЖурналBulletin of Experimental Biology and Medicine
Том170
Номер выпуска4
DOI
СостояниеОпубликовано - фев 2021

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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