Radiobromination of anti-HER2/neu/ErbB-2 monoclonal antibody using the p-isothiocyanatobenzene derivative of the [76Br]undecahydro-bromo-7, 8-dicarba-nido-undecaborate(1-) ion

Karl Johan Winberg, Mikael Persson, Per Uno Malmström, Stefan Sjöberg, Vladimir Tolmachev

Результат исследований: Материалы для журналаСтатьярецензирование

44 Цитирования (Scopus)

Аннотация

The monoclonal humanized anti-HER2 antibody trastuzumab was radiolabeled with the positron emitter 76Br (T12 =16.2 h). Indirect labeling was performed using the p-isothiocyanatobenzene derivative of the [76Br]undecahydro-bromo-7,8-dicarba-nido-undecaborate(1-) ( 76Br-NBI) as a precursor molecule. 76Br-NBI was prepared by bromination of the 7-(p-isothiocyanato-phenyl)dodecahydro-7,8-dicarba-nido- undecaborate(1-) ion (NBI) with a yield of 93-95% using Chloramine-T (CAT) as an oxidant. Coupling of radiobrominated NBI to antibody was performed without intermediate purification, in an "one pot" reaction. An overall labeling yield of 55.7 ± 4.8% (mean ± maximum error) was achieved when 300 μg of antibody was labeled. The label was stable in vitro in physiological and denaturing conditions. In a cell binding test, trastuzumab remained immunoreactive after labeling.

Язык оригиналаАнглийский
Страницы (с-по)425-433
Число страниц9
ЖурналNuclear Medicine and Biology
Том31
Номер выпуска4
DOI
СостояниеОпубликовано - мая 2004
Опубликовано для внешнего пользованияДа

ASJC Scopus subject areas

  • Molecular Medicine
  • Radiology Nuclear Medicine and imaging
  • Cancer Research

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