Purification of radiolabeled monoclonal antibodies to angiotensin-converting enzyme significantly improves specificity and efficacy of its targeting into the lung

H. Hiemisch, V. Gavrilyuk, Elena Nikolaevna Atochina, M. Slinkin, V. Torchilin, V. Muzykantov, S. Danilov

Результат исследований: Материалы для журналаСтатьярецензирование

8 Цитирования (Scopus)

Аннотация

The aim of this study was to improve the labeling/purification procedures for monoclonal antibody (MoAb) to angiotensin-converting enzyme (ACE). MoAb 9B9 was very stable upon iodination at a wide range of iodogen concentrations and incubation times, and was also very stable upon storage, indicating the high technological potential of this MoAb. Radiolabeled MoAb 9B9 was purified by (i) adsorption chromatography on cellulose, (ii) HPLC (gel filtration) and (iii) affinity chromatography on ACE-Sepharose. The best result was obtained with cellulose: specificity of MoAb 9B9 accumulation in the lung increased 2-fold. We conclude that the phenomenon of specific lung accumulation of MoAb 9B9 may serve as an ideal (convenient, cheap and technological) assay system for evaluation of monoclonal antibody modification and labeling.

Язык оригиналаАнглийский
Страницы (с-по)435-441
Число страниц7
ЖурналNuclear Medicine and Biology
Том20
Номер выпуска4
DOI
СостояниеОпубликовано - 1993
Опубликовано для внешнего пользованияДа

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Medicine
  • Radiology Nuclear Medicine and imaging

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