Production of the recombinant antimicrobial peptide UBI18-35 in Escherichia coli

Darya O. Ashcheulova, Lina V. Efimova, Aliaksandr Ya Lushchyk, Aliaksei V. Yantsevich, Alexander N. Baikov, Alexandra G. Pershina

Результат исследований: Материалы для журналаСтатья

4 Цитирования (Scopus)

Выдержка

Radiolabeled peptides derived from ubiquicidine (UBI) are of great interest for early and highly accurate scintigraphic detection and differentiation of infection and sterile inflammation. In the present work the recombinant antimicrobial peptide UBI18-35 - a fragment of the human natural cationic peptide ubiquicidine - was produced in Escherichia coli for the first time. The insoluble expression of the peptide in fusion with ketosteroid isomerase provided high yield, about 6 mg of UBI18-35 per liter. We developed an approach to produce the antimicrobial peptide UBI18-35, that encompasses inclusion body isolation and size exclusion chromatography. This method could be the basis for industrial biotechnological production of diagnostic system components that are in high demand.

Язык оригиналаАнглийский
Страницы (с-по)38-44
Число страниц7
ЖурналProtein Expression and Purification
Том143
DOI
СостояниеОпубликовано - 1 мар 2018

Отпечаток

Escherichia coli
Peptides
Ketosteroids
Isomerases
Inclusion Bodies
Body Size
Gel Chromatography
Inflammation
Infection

ASJC Scopus subject areas

  • Biotechnology

Цитировать

Production of the recombinant antimicrobial peptide UBI18-35 in Escherichia coli. / Ashcheulova, Darya O.; Efimova, Lina V.; Lushchyk, Aliaksandr Ya; Yantsevich, Aliaksei V.; Baikov, Alexander N.; Pershina, Alexandra G.

В: Protein Expression and Purification, Том 143, 01.03.2018, стр. 38-44.

Результат исследований: Материалы для журналаСтатья

Ashcheulova, Darya O. ; Efimova, Lina V. ; Lushchyk, Aliaksandr Ya ; Yantsevich, Aliaksei V. ; Baikov, Alexander N. ; Pershina, Alexandra G. / Production of the recombinant antimicrobial peptide UBI18-35 in Escherichia coli. В: Protein Expression and Purification. 2018 ; Том 143. стр. 38-44.
@article{405fbf7c4c4443fca5963719b5421b0a,
title = "Production of the recombinant antimicrobial peptide UBI18-35 in Escherichia coli",
abstract = "Radiolabeled peptides derived from ubiquicidine (UBI) are of great interest for early and highly accurate scintigraphic detection and differentiation of infection and sterile inflammation. In the present work the recombinant antimicrobial peptide UBI18-35 - a fragment of the human natural cationic peptide ubiquicidine - was produced in Escherichia coli for the first time. The insoluble expression of the peptide in fusion with ketosteroid isomerase provided high yield, about 6 mg of UBI18-35 per liter. We developed an approach to produce the antimicrobial peptide UBI18-35, that encompasses inclusion body isolation and size exclusion chromatography. This method could be the basis for industrial biotechnological production of diagnostic system components that are in high demand.",
keywords = "Antimicrobial peptides, Inclusion bodies, Ketosteroid isomerase, Size exclusion chromatography, Ubiquicidine",
author = "Ashcheulova, {Darya O.} and Efimova, {Lina V.} and Lushchyk, {Aliaksandr Ya} and Yantsevich, {Aliaksei V.} and Baikov, {Alexander N.} and Pershina, {Alexandra G.}",
year = "2018",
month = "3",
day = "1",
doi = "10.1016/j.pep.2017.10.011",
language = "English",
volume = "143",
pages = "38--44",
journal = "Protein Expression and Purification",
issn = "1046-5928",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Production of the recombinant antimicrobial peptide UBI18-35 in Escherichia coli

AU - Ashcheulova, Darya O.

AU - Efimova, Lina V.

AU - Lushchyk, Aliaksandr Ya

AU - Yantsevich, Aliaksei V.

AU - Baikov, Alexander N.

AU - Pershina, Alexandra G.

PY - 2018/3/1

Y1 - 2018/3/1

N2 - Radiolabeled peptides derived from ubiquicidine (UBI) are of great interest for early and highly accurate scintigraphic detection and differentiation of infection and sterile inflammation. In the present work the recombinant antimicrobial peptide UBI18-35 - a fragment of the human natural cationic peptide ubiquicidine - was produced in Escherichia coli for the first time. The insoluble expression of the peptide in fusion with ketosteroid isomerase provided high yield, about 6 mg of UBI18-35 per liter. We developed an approach to produce the antimicrobial peptide UBI18-35, that encompasses inclusion body isolation and size exclusion chromatography. This method could be the basis for industrial biotechnological production of diagnostic system components that are in high demand.

AB - Radiolabeled peptides derived from ubiquicidine (UBI) are of great interest for early and highly accurate scintigraphic detection and differentiation of infection and sterile inflammation. In the present work the recombinant antimicrobial peptide UBI18-35 - a fragment of the human natural cationic peptide ubiquicidine - was produced in Escherichia coli for the first time. The insoluble expression of the peptide in fusion with ketosteroid isomerase provided high yield, about 6 mg of UBI18-35 per liter. We developed an approach to produce the antimicrobial peptide UBI18-35, that encompasses inclusion body isolation and size exclusion chromatography. This method could be the basis for industrial biotechnological production of diagnostic system components that are in high demand.

KW - Antimicrobial peptides

KW - Inclusion bodies

KW - Ketosteroid isomerase

KW - Size exclusion chromatography

KW - Ubiquicidine

UR - http://www.scopus.com/inward/record.url?scp=85032341472&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85032341472&partnerID=8YFLogxK

U2 - 10.1016/j.pep.2017.10.011

DO - 10.1016/j.pep.2017.10.011

M3 - Article

AN - SCOPUS:85032341472

VL - 143

SP - 38

EP - 44

JO - Protein Expression and Purification

JF - Protein Expression and Purification

SN - 1046-5928

ER -