Production and functional characteristics of the recombinant targeted toxin based on the HER2-specific non-immunoglobulin scaffold

E. A. Sokolova, A. A. Schulga, O. A. Stremovskiy, I. V. Balalaeva, G. M. Proshkina, S. M. Deyev

Результат исследований: Материалы для журналаСтатья

1 Цитирования (Scopus)

Аннотация

Designing of new high-affinity protein compounds that can selectively destroy human cancer cells is a priority research area in biomedicine. In this work we report on the production procedure and describe functional characteristics of genetically encoded targeted toxin DARPin-PE40 for selective destruction of HER2-positive human cancer cells. HER2-specific non-immunoglobulin scaffold protein with ankyrin repeat was used as a targeting moiety. As a cytotoxic module, we used a fragment of the Pseudomonas exotoxin A. Using cytoplasmic expression in a bacterial system, we produced a monomelic protein with the yield of 180 mg per 1 L of culture medium. It was shown that targeted recombinant toxin possesses a considerable cytotoxic activity in vitro against HER2-positive cells (IC50 2 pM).

Язык оригиналаАнглийский
Страницы (с-по)429-434
Число страниц6
ЖурналBiologicheskie Membrany
Том33
Номер выпуска6
DOI
СостояниеОпубликовано - 2016

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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