Preparation of single-stranded antibodies to tick-borne encephalitis virus surface glycoprotein E

N. V. Tikunova, G. N. Nikolenko, Ye V. Protopopova, A. T. Kotelkin, V. B. Loktev, P. A. Belavin, N. A. Netesova, S. M. Deev, A. A. Ilyichev

Результат исследований: Материалы для журналаСтатьярецензирование

1 Цитирования (Scopus)

Аннотация

A single-stranded antibody gene scFv was designed on the base of cDNA fragments of genes coding for variable domains of heavy and light chains of MAb E6B to tick-borne encephalitis glycoprotein E. High production of stable soluble scFv was reproduced in Escherichia coli cells. Recombinant antibodies bound to antiidiotypical antibodies to initial MAb E6B and to recombinant virus protein E. Competitive analysis showed that single-stranded antibodies inhibited reaction between MAb E6B and protein E. These results confirm the formation of scFv with the original antigen-binding specificity towards tick-borne encephalitis virus glycoprotein E.

Язык оригиналаАнглийский
Страницы (с-по)12-15
Число страниц4
ЖурналVoprosy Virusologii
Том44
Номер выпуска1
СостояниеОпубликовано - 1999

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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