Polysaccharides PS1 and PS2 with a yield 1-2% were obtained from the above-ground part of three species of Saussurea: S. controversa DC., S. salicifolia L. and S. frolovii Ledeb. sequential extraction with water at 25 and 70 °C. PS1 free from protein impurities. A protein that is not removed by the Sevag method is co-extracted with PS2. All polysaccharides contain residues of uronic acids, the highest content found in PS1 and PS2 from S. contrversa. The molecular weights (Mw) of PS1 from S. contro-versa, S. salicifolia and S. frolovii were 448.13, 158.49, 64.03 kDa and PS2 - 101.82, 94.60, 225.42 kDa, respectively. Interspe-cific differences in the monosaccharide composition of polysaccharides were revealed. Galactose (Gal) and Arabinose (Ara) residues are major, and Rhamnose (Rha), Xylose (Xyl) and Mannose (Man) residues are minor components of the carbohydrate chains isolated by PS. PS1 S. salicifolia and S. frolovii and PS2 S. salicifolia do not contain endotoxins impurities and have a NO-activating effect on antigen-presenting cells (macrophages), significantly exceeding the effect of muramyl dipeptide.
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Plant Science
- Organic Chemistry