Pectin coatings on titanium alloy scaffolds produced by additive manufacturing: Promotion of human bone marrow stromal cell proliferation

Timothy E.L. Douglas, Ute Hempel, Jagoda Żydek, Alina Vladescu, Krzysztof Pietryga, Julia A.H. Kaeswurm, Maria Buchweitz, Roman A. Surmenev, Maria A. Surmeneva, Cosmin M. Cotrut, Andrey V. Koptyug, Elżbieta Pamuła

Результат исследований: Материалы для журналаСтатья

3 Цитирования (Scopus)

Выдержка

Ti6Al4V is a popular biomaterial for load-bearing implants for bone contact, which can be fabricated by additive manufacturing technologies. Their long-term success depends on their stable anchoring in surrounding bone, which in turn depends on formation of new bone tissue on the implant surface, for which adhesion and proliferation of bone-forming cells is a pre-requisite. Hence, surface coatings which promote cell adhesion and proliferation are desirable. Here, Ti6Al4V discs prepared by additive manufacturing (EBM) were coated with layers of pectins, calcium-binding polysaccharides derived from citrus (C) and apple (A), which also contained alkaline phosphatase (ALP), the enzyme responsible for mineralization of bone tissue. Adhesion and proliferation of human bone marrow stromal cells (hBMSC) were assessed. Proliferation after 7 days was increased by A-ALP coatings and, in particular, by C-ALP coatings. Cell morphology was similar on coated and uncoated samples. In conclusion, ALP-loaded pectin coatings promote hBMSC adhesion and proliferation.

Язык оригиналаАнглийский
Страницы (с-по)225-228
Число страниц4
ЖурналMaterials Letters
Том227
DOI
СостояниеОпубликовано - 15 сен 2018

Отпечаток

3D printers
bone marrow
Cell proliferation
titanium alloys
promotion
Titanium alloys
Scaffolds
phosphatases
bones
Bone
manufacturing
coatings
adhesion
Phosphatases
Coatings
Alkaline Phosphatase
Cell adhesion
cells
polysaccharides
Bearings (structural)

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

Цитировать

Pectin coatings on titanium alloy scaffolds produced by additive manufacturing : Promotion of human bone marrow stromal cell proliferation. / Douglas, Timothy E.L.; Hempel, Ute; Żydek, Jagoda; Vladescu, Alina; Pietryga, Krzysztof; Kaeswurm, Julia A.H.; Buchweitz, Maria; Surmenev, Roman A.; Surmeneva, Maria A.; Cotrut, Cosmin M.; Koptyug, Andrey V.; Pamuła, Elżbieta.

В: Materials Letters, Том 227, 15.09.2018, стр. 225-228.

Результат исследований: Материалы для журналаСтатья

Douglas, Timothy E.L. ; Hempel, Ute ; Żydek, Jagoda ; Vladescu, Alina ; Pietryga, Krzysztof ; Kaeswurm, Julia A.H. ; Buchweitz, Maria ; Surmenev, Roman A. ; Surmeneva, Maria A. ; Cotrut, Cosmin M. ; Koptyug, Andrey V. ; Pamuła, Elżbieta. / Pectin coatings on titanium alloy scaffolds produced by additive manufacturing : Promotion of human bone marrow stromal cell proliferation. В: Materials Letters. 2018 ; Том 227. стр. 225-228.
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abstract = "Ti6Al4V is a popular biomaterial for load-bearing implants for bone contact, which can be fabricated by additive manufacturing technologies. Their long-term success depends on their stable anchoring in surrounding bone, which in turn depends on formation of new bone tissue on the implant surface, for which adhesion and proliferation of bone-forming cells is a pre-requisite. Hence, surface coatings which promote cell adhesion and proliferation are desirable. Here, Ti6Al4V discs prepared by additive manufacturing (EBM) were coated with layers of pectins, calcium-binding polysaccharides derived from citrus (C) and apple (A), which also contained alkaline phosphatase (ALP), the enzyme responsible for mineralization of bone tissue. Adhesion and proliferation of human bone marrow stromal cells (hBMSC) were assessed. Proliferation after 7 days was increased by A-ALP coatings and, in particular, by C-ALP coatings. Cell morphology was similar on coated and uncoated samples. In conclusion, ALP-loaded pectin coatings promote hBMSC adhesion and proliferation.",
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AU - Vladescu, Alina

AU - Pietryga, Krzysztof

AU - Kaeswurm, Julia A.H.

AU - Buchweitz, Maria

AU - Surmenev, Roman A.

AU - Surmeneva, Maria A.

AU - Cotrut, Cosmin M.

AU - Koptyug, Andrey V.

AU - Pamuła, Elżbieta

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AB - Ti6Al4V is a popular biomaterial for load-bearing implants for bone contact, which can be fabricated by additive manufacturing technologies. Their long-term success depends on their stable anchoring in surrounding bone, which in turn depends on formation of new bone tissue on the implant surface, for which adhesion and proliferation of bone-forming cells is a pre-requisite. Hence, surface coatings which promote cell adhesion and proliferation are desirable. Here, Ti6Al4V discs prepared by additive manufacturing (EBM) were coated with layers of pectins, calcium-binding polysaccharides derived from citrus (C) and apple (A), which also contained alkaline phosphatase (ALP), the enzyme responsible for mineralization of bone tissue. Adhesion and proliferation of human bone marrow stromal cells (hBMSC) were assessed. Proliferation after 7 days was increased by A-ALP coatings and, in particular, by C-ALP coatings. Cell morphology was similar on coated and uncoated samples. In conclusion, ALP-loaded pectin coatings promote hBMSC adhesion and proliferation.

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