Participation of opioid receptors in the cytoprotective effect of chronic normobaric hypoxia

N. V. Naryzhnaya, I. Khaliulin, Y. B. Lishmanov, M. Saadeh Suleiman, S. Y. Tsibulnikov, F. Kolar, L. N. Maslov

Результат исследований: Материалы для журналаСтатьярецензирование

4 Цитирования (Scopus)


We studied the role of the δ, μ, and κ opioid receptor (OR) subtypes in the cardioprotective effect of chronic continuous normobaric hypoxia (CNH) in the model of acuteanoxia/ reoxygenation of isolated cardiomyocytes. Adaptation of rats to CNH was performed by their exposure to atmosphere containing 12 % of O 2 for 21 days. Anoxia/reoxygenation of cardiomyocytes isolated from normoxic control rats caused the death of 51 % of cells and lactate dehydrogenase (LDH) release. Adaptation of rats to CNH resulted in the anoxia/reoxygenation-induced cardiomyocyte death of only 38 %, and reduced the LDH release. Pre-incubation of the cells with either the non-selective OR blocker naloxone (300 nM/l), the δ OR antagonist TIPP(ψ) (30 nM/l), the selective δ 2 OR antagonist naltriben (1 nM/l) or the μ OR antagonist CTAP (100 nM/l) for 25 minutes before anoxia abolished the reduction of cell death and LDH release afforded by CNH. The antagonist of δ 1 OR BNTX (1 nM/l) or the κ OR antagonist nor-binaltorphimine (3 nM/l) did not influence the cytoprotective effects of CNH. Taken together, the cytoprotective effect of CNH is associated with the activation of the δ 2 and μ OR localized on cardiomyocytes.

Язык оригиналаАнглийский
Страницы (с-по)245-253
Число страниц9
ЖурналPhysiological Research
Номер выпуска2
СостояниеОпубликовано - 1 янв 2019

ASJC Scopus subject areas

  • Physiology

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