Osteogenic potential of mesenchymal stem cells from bone marrow in situ: Role of physicochemical properties of artificial surfaces

Igor Albertovich Khlusov, A. V. Karlov, Yu P. Sharkeev, V. F. Pichugin, Yu P. Kolobov, G. A. Shashkina, M. B. Ivanov, E. V. Legostaeva, G. T. Sukhikh

Результат исследований: Материалы для журналаСтатья

21 Цитирования (Scopus)

Выдержка

Correlation analysis demonstrated the role of inorganic parameters of the surfaces of calcium phosphate materials in the regulation of osteogenic differentiation of mesenchymal precursors. The progenitor stromal cells were isolated from syngeneic bone marrow immobilized in vitro on calcium phosphate surfaces with different structure, phasic, and elemental composition. After 45 days of subcutaneous ectopic osteogenesis in BALB/c mice, the tissues grown on these matrixes were characterized histologically. It was found that adhesion of bone marrow cells is the initial stage determining their future proliferation (conduction) over the artificial surface and the area of formed tissue plate. The success of histogenesis depends on surface roughness. The optimal roughness class was 4-5 (Russian State Standards), which enables differentiation of progenitor stromal cells under the specific microenvironmental conditions into the connective and adipose tissue cells. Differentiation of the progenitor cells into the stromal cells producing the hemopoiesis-inducing microenvironment also takes place in the foci of active hemopoiesis. Induction of osteogenic potential of the stromal precursors (osteoinduction) is determined by the ratio between calcium and phosphate atoms in surface coatings. In our experimental system, osteogenic differentiation of stromal mechanocytes was blocked only at Ca/P<0.5.

Язык оригиналаАнглийский
Страницы (с-по)144-152
Число страниц9
ЖурналBulletin of Experimental Biology and Medicine
Том140
Номер выпуска1
DOI
СостояниеОпубликовано - 1 июл 2005

Отпечаток

Surface Properties
Stromal Cells
Stem cells
Mesenchymal Stromal Cells
Bone
Stem Cells
Bone Marrow
Tissue
Connective Tissue Cells
Surface roughness
Osteogenesis
Bone Marrow Cells
Adipose Tissue
Adhesion
Cells
Coatings
Atoms
calcium phosphate
Chemical analysis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Цитировать

Osteogenic potential of mesenchymal stem cells from bone marrow in situ : Role of physicochemical properties of artificial surfaces. / Khlusov, Igor Albertovich; Karlov, A. V.; Sharkeev, Yu P.; Pichugin, V. F.; Kolobov, Yu P.; Shashkina, G. A.; Ivanov, M. B.; Legostaeva, E. V.; Sukhikh, G. T.

В: Bulletin of Experimental Biology and Medicine, Том 140, № 1, 01.07.2005, стр. 144-152.

Результат исследований: Материалы для журналаСтатья

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abstract = "Correlation analysis demonstrated the role of inorganic parameters of the surfaces of calcium phosphate materials in the regulation of osteogenic differentiation of mesenchymal precursors. The progenitor stromal cells were isolated from syngeneic bone marrow immobilized in vitro on calcium phosphate surfaces with different structure, phasic, and elemental composition. After 45 days of subcutaneous ectopic osteogenesis in BALB/c mice, the tissues grown on these matrixes were characterized histologically. It was found that adhesion of bone marrow cells is the initial stage determining their future proliferation (conduction) over the artificial surface and the area of formed tissue plate. The success of histogenesis depends on surface roughness. The optimal roughness class was 4-5 (Russian State Standards), which enables differentiation of progenitor stromal cells under the specific microenvironmental conditions into the connective and adipose tissue cells. Differentiation of the progenitor cells into the stromal cells producing the hemopoiesis-inducing microenvironment also takes place in the foci of active hemopoiesis. Induction of osteogenic potential of the stromal precursors (osteoinduction) is determined by the ratio between calcium and phosphate atoms in surface coatings. In our experimental system, osteogenic differentiation of stromal mechanocytes was blocked only at Ca/P<0.5.",
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AU - Pichugin, V. F.

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AU - Shashkina, G. A.

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AU - Legostaeva, E. V.

AU - Sukhikh, G. T.

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