Osteoblastic differentiation of mesenchymal stem cells by mumie extract

Cho Rok Jung, Igor A. Schepetkin, Sang B. Woo, Andrey Ivanovich Khlebnikov, Byoung S. Kwon

Результат исследований: Материалы для журналаСтатья

10 Цитирования (Scopus)

Аннотация

Mumie, a plant humus matter from rocks, is known as anabolic and a stimulator of bone regeneration in the Russian and Indian systems of health and medicine. The water-soluble fraction of mumie from Uzbekistan was characterized using 1H NMR and infrared spectroscopic methods. The mumie extract has been investigated for its effect on osteoblastic differentiation in cell culture assays of human and murine mesenchymal stem cells. The calcium deposition and expression of alkaline phosphatase, osteocalcin, core binding factor 1 (Cbfa1), and ERK have been studied. During the 14-day assay period, human bone marrow mesenchymal stem cells (hMSCs) and human fetal osteoblasts cultured with mumie (3-5 μg/ml) underwent a dramatic change in cellular morphology, which was accompanied by a significant increase in alkaline phosphatase activity, calcium deposition, and osteocalcin expression. The expression of core binding factor 1 and ERK were enhanced in hMSCs and murine pluripotent mesenchymal precursor cell line C2C12. Dose-dependent decrease in TRAP-positive multinucleated cell formation from macrophage-like cells RAW 264.7 was observed with increasing concentration of mumie in the presence of RANKL (40 ng/ml) and PD98059 (10 μM), a specific inhibitor of ERK activity. The data suggest that mumie is a potent stimulator of osteoblastic differentiation of mesenchymal stem cells and inhibitor of osteoclastogenesis, hence it maybe of clinical benefit in the treatment for osteoporosis in human.

Язык оригиналаАнглийский
Страницы (с-по)122-133
Число страниц12
ЖурналDrug Development Research
Том57
Номер выпуска3
DOI
СостояниеОпубликовано - 1 ноя 2002
Опубликовано для внешнего пользованияДа

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ASJC Scopus subject areas

  • Organic Chemistry
  • Drug Discovery
  • Pharmacology

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