Molecular Identification and Characterization of hRPC11, the Smallest Subunit Specific for Human RNA Polymerase III

G. V. Shpakovski, E. N. Lebedenko

Результат исследований: Материалы для журналаСтатьярецензирование

Аннотация

Full-length copies of cDNA of the hRPC11 gene encoding the smallest specific subunit of nuclear RNA polymerase III were identified among human transcripts with the use of the RT-PCR technique. The cloning of the first orthologue of the subunit RPC11 from a multicellular organism and the comparison of subunit hRPC11 of Homo sapiens (108 aa; Mr 12.3 kDa; pI 8.05) deduced from the cDNA primary structure with the homologous components of RNA polymerase III from Saccharomyces cerevisiae and Schizosaccharomyces pombe revealed the most important functional domains: a Zn-binding motif of the classic type (CxxCx16-17CxxC) at the N-terminal region, and two extended regions of homology (KEVDDVLGG and RSADEPM) in the central and C-terminal parts of the molecule, respectively. The C-terminus of the RPC11 subunits is highly homologous to the unique zinc ribbon of the elongation factor TFIIS, which suggests a role for this subunit in the elongation or termination of RNA synthesis.

Язык оригиналаАнглийский
Страницы (с-по)880
Число страниц1
ЖурналBioorganicheskaya Khimiya
Том24
Номер выпуска11
СостояниеОпубликовано - 1998
Опубликовано для внешнего пользованияДа

ASJC Scopus subject areas

  • Medicine(all)

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