TY - JOUR
T1 - Investigation of electrochemical properties of silver nanoparticles conjugated with antibodies to tick-borne encephalitis for development of electrochemical immunosensor
AU - Khristunova, Ekaterina P.
AU - Kratochvil, Bohumil
AU - Dorozhko, Elena V.
AU - Korotkova, Elena I.
N1 - Publisher Copyright:
© 2020, Ivanovo State University of Chemistry and Technology.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020
Y1 - 2020
N2 - In the past decade, interest to electrochemical assays have grown for determination anti-bodies in clinical and biological samples, where instead of the enzyme label metal nanoparticle is used. Such types of electrochemical immunosensors represent an upcoming trend in analytical chemistry. In this work, the electrochemical behavior of silver nanoparticles conjugated with anti-bodies to tick-borne encephalitis virus at different stages of electrode modification is investigated. Silver nanoparticles were obtained by chemical reduction from silver nitrate. Passive adsorption was chosen as a strategy for binding silver nanoparticles to antibodies to tick-borne encephalitis virus. The optimal ratio of antibodies to tick-borne encephalitis virus (4.5 IU ml-1) in a solution of silver nanoparticles [1 mmol l-1 AgNO3] was found experimentally. The development of an electro-chemical immunosensor was based on indirect, non-competitive format of enzyme-linked immuno-sorbent assay. The electrochemical situation on the electrode surface after modification steps was demonstrated by cyclic voltammetry using a standard redox pair [Fe(CN)6]3-/[Fe(CN)6]4-. Glassy carbon electrode with electrochemically deposited gold nanoparticles was used as a platform for immobilization of biological material. It has been established that after sensitization of the electrode with antigen, passivation of the surface occurs. The voltammetric signal was recorded through the detection of silver ions oxidation on unmodified glassy carbon electrode under the working condi-tions: scan rate 0.1 V s-1, potential accumulation-0.8 V, accumulation time 60 s. Thus, this work paves the way for a new alternative method for monitoring antibodies to tick-borne encephalitis virus in biological fluids.
AB - In the past decade, interest to electrochemical assays have grown for determination anti-bodies in clinical and biological samples, where instead of the enzyme label metal nanoparticle is used. Such types of electrochemical immunosensors represent an upcoming trend in analytical chemistry. In this work, the electrochemical behavior of silver nanoparticles conjugated with anti-bodies to tick-borne encephalitis virus at different stages of electrode modification is investigated. Silver nanoparticles were obtained by chemical reduction from silver nitrate. Passive adsorption was chosen as a strategy for binding silver nanoparticles to antibodies to tick-borne encephalitis virus. The optimal ratio of antibodies to tick-borne encephalitis virus (4.5 IU ml-1) in a solution of silver nanoparticles [1 mmol l-1 AgNO3] was found experimentally. The development of an electro-chemical immunosensor was based on indirect, non-competitive format of enzyme-linked immuno-sorbent assay. The electrochemical situation on the electrode surface after modification steps was demonstrated by cyclic voltammetry using a standard redox pair [Fe(CN)6]3-/[Fe(CN)6]4-. Glassy carbon electrode with electrochemically deposited gold nanoparticles was used as a platform for immobilization of biological material. It has been established that after sensitization of the electrode with antigen, passivation of the surface occurs. The voltammetric signal was recorded through the detection of silver ions oxidation on unmodified glassy carbon electrode under the working condi-tions: scan rate 0.1 V s-1, potential accumulation-0.8 V, accumulation time 60 s. Thus, this work paves the way for a new alternative method for monitoring antibodies to tick-borne encephalitis virus in biological fluids.
KW - Bioconjugate
KW - Electrochemical immunosensor
KW - Silver nanoparticles
KW - Tick-borne encephalitis
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U2 - 10.6060/ivkkt.20206304.6160
DO - 10.6060/ivkkt.20206304.6160
M3 - Article
AN - SCOPUS:85088024563
VL - 63
SP - 28
EP - 33
JO - IZVESTIYA VYSSHIKH UCHEBNYKH ZAVEDENII KHIMIYA I KHIMICHESKAYA TEKHNOLOGIYA
JF - IZVESTIYA VYSSHIKH UCHEBNYKH ZAVEDENII KHIMIYA I KHIMICHESKAYA TEKHNOLOGIYA
SN - 0579-2991
IS - 4
ER -