Interaction study of methyl violet 2B with DNA and voltammetric determination of DNA in aqueous solutions

Eva Horakova, Vlastimil Vyskocil, Jiri Barek

Результат исследований: Материалы для журналаСтатья

2 Цитирования (Scopus)

Выдержка

Differential pulse voltammetry, cyclic voltammetry, and chronocoulometry at a hanging mercury drop electrode were used for the study of the interaction of methyl violet 2B (MV) with double-stranded DNA in 0.1 mol dm-3 acetate buffer pH 4.0. The mechanism of the electrochemical reduction of MV was also investigated, using cyclic voltammetry at a hanging mercury drop electrode. UV-Vis spectrophotometry was used for the investigation of the interaction as well. The kinetic parameters of the reduction of MV and the DNA-MV complex and the thermodynamic parameters of the formed DNA-MV complex were calculated. Moreover, the calibration dependence of the peak current of MV on the concentration of DNA in acetate buffer was constructed to be used for the indirect determination of DNA based on the decreasing peak current of MV with increasing concentration of DNA in the measured solution.

Язык оригиналаАнглийский
Страницы (с-по)119-126
Число страниц8
ЖурналMonatshefte fur Chemie
Том147
Номер выпуска1
DOI
СостояниеОпубликовано - 1 янв 2016
Опубликовано для внешнего пользованияДа

Отпечаток

Gentian Violet
DNA
Mercury
Cyclic voltammetry
Buffers
Acetates
Electrodes
Spectrophotometry
Kinetic parameters
Thermodynamics
Calibration

ASJC Scopus subject areas

  • Chemistry(all)

Цитировать

Interaction study of methyl violet 2B with DNA and voltammetric determination of DNA in aqueous solutions. / Horakova, Eva; Vyskocil, Vlastimil; Barek, Jiri.

В: Monatshefte fur Chemie, Том 147, № 1, 01.01.2016, стр. 119-126.

Результат исследований: Материалы для журналаСтатья

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abstract = "Differential pulse voltammetry, cyclic voltammetry, and chronocoulometry at a hanging mercury drop electrode were used for the study of the interaction of methyl violet 2B (MV) with double-stranded DNA in 0.1 mol dm-3 acetate buffer pH 4.0. The mechanism of the electrochemical reduction of MV was also investigated, using cyclic voltammetry at a hanging mercury drop electrode. UV-Vis spectrophotometry was used for the investigation of the interaction as well. The kinetic parameters of the reduction of MV and the DNA-MV complex and the thermodynamic parameters of the formed DNA-MV complex were calculated. Moreover, the calibration dependence of the peak current of MV on the concentration of DNA in acetate buffer was constructed to be used for the indirect determination of DNA based on the decreasing peak current of MV with increasing concentration of DNA in the measured solution.",
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AU - Barek, Jiri

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