Immobilization of fluorescent protein TagGFP2 on Fe3O4-based magnetic nanoparticles

A. M. Demin, M. S. Valova, A. G. Pershina, V. P. Krasnov

Результат исследований: Материалы для журналаСтатья

1 цитирование (Scopus)

Выдержка

The immobilization of fluorescent protein TagGFP2 on Fe3O4-based magnetic nano particles (MNPs), preliminarily functionalized with 3-aminopropylsilane and N-(phosphonomethyl) iminodiacetic acid (PMIDA), was studied. Protein binding to MNPs depending on the nature of the surface functional groups, the medium, and the presence of a coupling agent, namely, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDC), was studied using fluorimetry, UV and IR spectroscopy. It was shown that protein immobilization can occur by both non-covalent and covalent binding with surface groups of MNPs. The best results (up to 150 μg of TagGFP2 per 1 mg of MNPs) were obtained for MNPs preliminarily functionalized with PMIDA and using EDC. The results of this study can be used for the design of imaging agents having both magnetic and optical properties to be applied in biomedicine.

Язык оригиналаАнглийский
Страницы (с-по)1088-1095
Число страниц8
ЖурналRussian Chemical Bulletin
Том68
Номер выпуска5
DOI
СостояниеОпубликовано - 1 мая 2019

Отпечаток

Ethyldimethylaminopropyl Carbodiimide
Nanoparticles
Coupling agents
Ultraviolet spectroscopy
Functional groups
Infrared spectroscopy
Magnetic properties
Proteins
Optical properties
Imaging techniques
iminodiacetic acid
Protein Binding
aminopropylsilane

ASJC Scopus subject areas

  • Chemistry(all)

Цитировать

Immobilization of fluorescent protein TagGFP2 on Fe3O4-based magnetic nanoparticles. / Demin, A. M.; Valova, M. S.; Pershina, A. G.; Krasnov, V. P.

В: Russian Chemical Bulletin, Том 68, № 5, 01.05.2019, стр. 1088-1095.

Результат исследований: Материалы для журналаСтатья

@article{adfefa33f55548eab01431c433a4b2bb,
title = "Immobilization of fluorescent protein TagGFP2 on Fe3O4-based magnetic nanoparticles",
abstract = "The immobilization of fluorescent protein TagGFP2 on Fe3O4-based magnetic nano particles (MNPs), preliminarily functionalized with 3-aminopropylsilane and N-(phosphonomethyl) iminodiacetic acid (PMIDA), was studied. Protein binding to MNPs depending on the nature of the surface functional groups, the medium, and the presence of a coupling agent, namely, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDC), was studied using fluorimetry, UV and IR spectroscopy. It was shown that protein immobilization can occur by both non-covalent and covalent binding with surface groups of MNPs. The best results (up to 150 μg of TagGFP2 per 1 mg of MNPs) were obtained for MNPs preliminarily functionalized with PMIDA and using EDC. The results of this study can be used for the design of imaging agents having both magnetic and optical properties to be applied in biomedicine.",
keywords = "fluorimetry, magnetic nanoparticles, TagGFP2, UV spectroscopy",
author = "Demin, {A. M.} and Valova, {M. S.} and Pershina, {A. G.} and Krasnov, {V. P.}",
year = "2019",
month = "5",
day = "1",
doi = "10.1007/s11172-019-2524-1",
language = "English",
volume = "68",
pages = "1088--1095",
journal = "Russian Chemical Bulletin",
issn = "1066-5285",
publisher = "Springer New York",
number = "5",

}

TY - JOUR

T1 - Immobilization of fluorescent protein TagGFP2 on Fe3O4-based magnetic nanoparticles

AU - Demin, A. M.

AU - Valova, M. S.

AU - Pershina, A. G.

AU - Krasnov, V. P.

PY - 2019/5/1

Y1 - 2019/5/1

N2 - The immobilization of fluorescent protein TagGFP2 on Fe3O4-based magnetic nano particles (MNPs), preliminarily functionalized with 3-aminopropylsilane and N-(phosphonomethyl) iminodiacetic acid (PMIDA), was studied. Protein binding to MNPs depending on the nature of the surface functional groups, the medium, and the presence of a coupling agent, namely, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDC), was studied using fluorimetry, UV and IR spectroscopy. It was shown that protein immobilization can occur by both non-covalent and covalent binding with surface groups of MNPs. The best results (up to 150 μg of TagGFP2 per 1 mg of MNPs) were obtained for MNPs preliminarily functionalized with PMIDA and using EDC. The results of this study can be used for the design of imaging agents having both magnetic and optical properties to be applied in biomedicine.

AB - The immobilization of fluorescent protein TagGFP2 on Fe3O4-based magnetic nano particles (MNPs), preliminarily functionalized with 3-aminopropylsilane and N-(phosphonomethyl) iminodiacetic acid (PMIDA), was studied. Protein binding to MNPs depending on the nature of the surface functional groups, the medium, and the presence of a coupling agent, namely, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDC), was studied using fluorimetry, UV and IR spectroscopy. It was shown that protein immobilization can occur by both non-covalent and covalent binding with surface groups of MNPs. The best results (up to 150 μg of TagGFP2 per 1 mg of MNPs) were obtained for MNPs preliminarily functionalized with PMIDA and using EDC. The results of this study can be used for the design of imaging agents having both magnetic and optical properties to be applied in biomedicine.

KW - fluorimetry

KW - magnetic nanoparticles

KW - TagGFP2

KW - UV spectroscopy

UR - http://www.scopus.com/inward/record.url?scp=85067050138&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85067050138&partnerID=8YFLogxK

U2 - 10.1007/s11172-019-2524-1

DO - 10.1007/s11172-019-2524-1

M3 - Article

AN - SCOPUS:85067050138

VL - 68

SP - 1088

EP - 1095

JO - Russian Chemical Bulletin

JF - Russian Chemical Bulletin

SN - 1066-5285

IS - 5

ER -