Hemozoin "knobs" in Opisthorchis felineus infected liver

Alexandra G. Pershina, Irina V. Saltykova, Vladimir V. Ivanov, Ekaterina A. Perina, Alexander M. Demin, Oleg B. Shevelev, Irina I. Buzueva, Anton K. Gutakovskii, Sergey V. Vtorushin, Ilya N. Ganebnykh, Victor P. Krasnov, Alexey E. Sazonov, Ludmila M. Ogorodova

Результат исследований: Материалы для журналаСтатья

10 Цитирования (Scopus)

Выдержка

Background: Hemozoin is the pigment produced by some blood-feeding parasites. It demonstrates high diagnostic and therapeutic potential. In this work the formation of co-called hemozoin "knobs" - the bile duct ectasia filled up by hemozoin pigment - in Opisthorhis felineus infected hamster liver has been observed. Methods: The O. felineus infected liver was examined by histological analysis and magnetic resonance imaging (MRI). The pigment hemozoin was identified by Fourier transform infrared spectroscopy and high resolution electrospray ionization mass spectrometry analysis. Hemozoin crystals were characterised by high resolution transmission electron microscopy. Results: Hemozoin crystals produced by O. felineus have average length 403 nm and the length-to-width ratio equals 2.0. The regurgitation of hemozoin from parasitic fluke during infection leads to formation of bile duct ectasia. The active release of hemozoin from O. felineus during in vitro incubation has also been evidenced. It has been shown that the hemozoin knobs can be detected by magnetic resonance imaging. Conclusions: In the paper for the first time the characterisation of hemozoin pigment extracted from liver fluke O. felineus has been conducted. The role of hemozoin in the modification of immune response by opisthorchiasis is assumed.

Язык оригиналаАнглийский
Номер статьи459
ЖурналParasites and Vectors
Том8
Номер выпуска1
DOI
СостояниеОпубликовано - 17 сен 2015

Отпечаток

Opisthorchis
Liver
Pathologic Dilatations
Bile Ducts
hemozoin
Opisthorchiasis
Magnetic Resonance Imaging
Fasciola hepatica
Trematoda
Electrospray Ionization Mass Spectrometry
Fourier Transform Infrared Spectroscopy
Transmission Electron Microscopy
Cricetinae

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Цитировать

Pershina, A. G., Saltykova, I. V., Ivanov, V. V., Perina, E. A., Demin, A. M., Shevelev, O. B., ... Ogorodova, L. M. (2015). Hemozoin "knobs" in Opisthorchis felineus infected liver. Parasites and Vectors, 8(1), [459]. https://doi.org/10.1186/s13071-015-1061-5

Hemozoin "knobs" in Opisthorchis felineus infected liver. / Pershina, Alexandra G.; Saltykova, Irina V.; Ivanov, Vladimir V.; Perina, Ekaterina A.; Demin, Alexander M.; Shevelev, Oleg B.; Buzueva, Irina I.; Gutakovskii, Anton K.; Vtorushin, Sergey V.; Ganebnykh, Ilya N.; Krasnov, Victor P.; Sazonov, Alexey E.; Ogorodova, Ludmila M.

В: Parasites and Vectors, Том 8, № 1, 459, 17.09.2015.

Результат исследований: Материалы для журналаСтатья

Pershina, AG, Saltykova, IV, Ivanov, VV, Perina, EA, Demin, AM, Shevelev, OB, Buzueva, II, Gutakovskii, AK, Vtorushin, SV, Ganebnykh, IN, Krasnov, VP, Sazonov, AE & Ogorodova, LM 2015, 'Hemozoin "knobs" in Opisthorchis felineus infected liver', Parasites and Vectors, том. 8, № 1, 459. https://doi.org/10.1186/s13071-015-1061-5
Pershina AG, Saltykova IV, Ivanov VV, Perina EA, Demin AM, Shevelev OB и соавт. Hemozoin "knobs" in Opisthorchis felineus infected liver. Parasites and Vectors. 2015 Сент. 17;8(1). 459. https://doi.org/10.1186/s13071-015-1061-5
Pershina, Alexandra G. ; Saltykova, Irina V. ; Ivanov, Vladimir V. ; Perina, Ekaterina A. ; Demin, Alexander M. ; Shevelev, Oleg B. ; Buzueva, Irina I. ; Gutakovskii, Anton K. ; Vtorushin, Sergey V. ; Ganebnykh, Ilya N. ; Krasnov, Victor P. ; Sazonov, Alexey E. ; Ogorodova, Ludmila M. / Hemozoin "knobs" in Opisthorchis felineus infected liver. В: Parasites and Vectors. 2015 ; Том 8, № 1.
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AU - Pershina, Alexandra G.

AU - Saltykova, Irina V.

AU - Ivanov, Vladimir V.

AU - Perina, Ekaterina A.

AU - Demin, Alexander M.

AU - Shevelev, Oleg B.

AU - Buzueva, Irina I.

AU - Gutakovskii, Anton K.

AU - Vtorushin, Sergey V.

AU - Ganebnykh, Ilya N.

AU - Krasnov, Victor P.

AU - Sazonov, Alexey E.

AU - Ogorodova, Ludmila M.

PY - 2015/9/17

Y1 - 2015/9/17

N2 - Background: Hemozoin is the pigment produced by some blood-feeding parasites. It demonstrates high diagnostic and therapeutic potential. In this work the formation of co-called hemozoin "knobs" - the bile duct ectasia filled up by hemozoin pigment - in Opisthorhis felineus infected hamster liver has been observed. Methods: The O. felineus infected liver was examined by histological analysis and magnetic resonance imaging (MRI). The pigment hemozoin was identified by Fourier transform infrared spectroscopy and high resolution electrospray ionization mass spectrometry analysis. Hemozoin crystals were characterised by high resolution transmission electron microscopy. Results: Hemozoin crystals produced by O. felineus have average length 403 nm and the length-to-width ratio equals 2.0. The regurgitation of hemozoin from parasitic fluke during infection leads to formation of bile duct ectasia. The active release of hemozoin from O. felineus during in vitro incubation has also been evidenced. It has been shown that the hemozoin knobs can be detected by magnetic resonance imaging. Conclusions: In the paper for the first time the characterisation of hemozoin pigment extracted from liver fluke O. felineus has been conducted. The role of hemozoin in the modification of immune response by opisthorchiasis is assumed.

AB - Background: Hemozoin is the pigment produced by some blood-feeding parasites. It demonstrates high diagnostic and therapeutic potential. In this work the formation of co-called hemozoin "knobs" - the bile duct ectasia filled up by hemozoin pigment - in Opisthorhis felineus infected hamster liver has been observed. Methods: The O. felineus infected liver was examined by histological analysis and magnetic resonance imaging (MRI). The pigment hemozoin was identified by Fourier transform infrared spectroscopy and high resolution electrospray ionization mass spectrometry analysis. Hemozoin crystals were characterised by high resolution transmission electron microscopy. Results: Hemozoin crystals produced by O. felineus have average length 403 nm and the length-to-width ratio equals 2.0. The regurgitation of hemozoin from parasitic fluke during infection leads to formation of bile duct ectasia. The active release of hemozoin from O. felineus during in vitro incubation has also been evidenced. It has been shown that the hemozoin knobs can be detected by magnetic resonance imaging. Conclusions: In the paper for the first time the characterisation of hemozoin pigment extracted from liver fluke O. felineus has been conducted. The role of hemozoin in the modification of immune response by opisthorchiasis is assumed.

KW - Bile duct ectasia

KW - Hemozoin

KW - Magnetic resonance imaging (MRI)

KW - Opisthorchis felineus

KW - Transmission electron microscopy (TEM)

KW - Trematode

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