Hemozoin "knobs" in Opisthorchis felineus infected liver

Alexandra G. Pershina, Irina V. Saltykova, Vladimir V. Ivanov, Ekaterina A. Perina, Alexander M. Demin, Oleg B. Shevelev, Irina I. Buzueva, Anton K. Gutakovskii, Sergey V. Vtorushin, Ilya N. Ganebnykh, Victor P. Krasnov, Alexey E. Sazonov, Ludmila M. Ogorodova

Результат исследований: Материалы для журналаСтатьярецензирование

10 Цитирования (Scopus)

Аннотация

Background: Hemozoin is the pigment produced by some blood-feeding parasites. It demonstrates high diagnostic and therapeutic potential. In this work the formation of co-called hemozoin "knobs" - the bile duct ectasia filled up by hemozoin pigment - in Opisthorhis felineus infected hamster liver has been observed. Methods: The O. felineus infected liver was examined by histological analysis and magnetic resonance imaging (MRI). The pigment hemozoin was identified by Fourier transform infrared spectroscopy and high resolution electrospray ionization mass spectrometry analysis. Hemozoin crystals were characterised by high resolution transmission electron microscopy. Results: Hemozoin crystals produced by O. felineus have average length 403 nm and the length-to-width ratio equals 2.0. The regurgitation of hemozoin from parasitic fluke during infection leads to formation of bile duct ectasia. The active release of hemozoin from O. felineus during in vitro incubation has also been evidenced. It has been shown that the hemozoin knobs can be detected by magnetic resonance imaging. Conclusions: In the paper for the first time the characterisation of hemozoin pigment extracted from liver fluke O. felineus has been conducted. The role of hemozoin in the modification of immune response by opisthorchiasis is assumed.

Язык оригиналаАнглийский
Номер статьи459
ЖурналParasites and Vectors
Том8
Номер выпуска1
DOI
СостояниеОпубликовано - 17 сен 2015

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

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