Group-selective immunoassay

Sergey A. Yazynin, Sergey M. Deyev

Результат исследований: Материалы для журналаСтатьярецензирование

2 Цитирования (Scopus)

Аннотация

A new solid-phase immunoassay technique has been applied for anti-digoxin monoclonal antibodies (Mabs) detection. At the first assay step, anti-digoxin Mabs (IgG1, Kaff = 9.2 × 109 M-1) were bound to a specially prepared immunosorbent, the microtiter plates coated with digoxin-human serum albumin conjugate (Dig-HSA), in which free amino groups were protected by a glutaraldehyde cross-linking modification. The modification did not essentially influence the antibody-binding capacity of the immunosorbent. After antigen-antibody reaction, free amino groups were located only on the anti-digoxin Mabs, bound to chemically modified immunosorbent. At the second assay step, free amino groups of anti-digoxin Mabs were biotinylated by N-hydroxysuccinimide-biotin ester. Then the biotin residues were detected by the streptavidin-peroxidase conjugate. The method does not require second labeled antibodies and may be used for anti-hapten hybridoma screening.

Язык оригиналаАнглийский
Страницы (с-по)235-239
Число страниц5
ЖурналImmunology Letters
Том41
Номер выпуска2-3
DOI
СостояниеОпубликовано - 1994
Опубликовано для внешнего пользованияДа

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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