Genetically encoded immunophotosensitizer

E. O. Serebrovskaya, O. A. Stremovsky, D. M. Chudakov, K. A. Lukyanov, S. M. Deyev

Результат исследований: Материалы для журналаСтатья

1 Цитирования (Scopus)

Аннотация

Conjugates of antibodies with photosensitizers are successfully used for the targeted killing of cancer cells bearing particular surface markers by the method known as photoimmunotherapy. However, the chemical conjugation of photosensitizers with antibodies poses a number of problems. Among these are a low reproducibility, aggregation, and the presence of impurities of the unconjugated photosensitizer. Here we describe a method of designing a fully genetically encoded immunophotosensitizer, which consists of the anti-HER2/neu miniantibody 4D5scFv as a targeting molecule and the phototoxic fluorescent protein KillerRed as a photosensitizing molecule. Both domains in the recombinant protein retained their functional properties: a high affinity for the HER2/neu antigen and phototoxicity. The recombinant protein 4D5scFv-KillerRed showed a high specificity for HER2/neu-overexpressing cancer cells and effectively lowered their viability upon irradiation.

Язык оригиналаАнглийский
Страницы (с-по)123-129
Число страниц7
ЖурналRussian Journal of Bioorganic Chemistry
Том37
Номер выпуска1
DOI
СостояниеОпубликовано - янв 2011
Опубликовано для внешнего пользованияДа

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry

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  • Цитировать

    Serebrovskaya, E. O., Stremovsky, O. A., Chudakov, D. M., Lukyanov, K. A., & Deyev, S. M. (2011). Genetically encoded immunophotosensitizer. Russian Journal of Bioorganic Chemistry, 37(1), 123-129. https://doi.org/10.1134/S1068162011010134