Flavoprotein miniSOG BRET-induced cytotoxicity depends on its intracellular localization

E. I. Shramova, G. M. Proshkina, S. M. Deyev, R. V. Petrov

Результат исследований: Материалы для журналаСтатья

7 Цитирования (Scopus)

Аннотация

It is proposed to use the bioluminescent resonance energy transfer to solve the problem of creating the internal light sources in photodynamic therapy of cancer. Energy donor in the developed system is the oxidized form of the luciferase NanoLuc substrate furimamide, and acceptor is the phototoxic fluorescent protein miniSOG. It is shown that, in the proposed system, the photoinduced cytotoxicity of flavoprotein miniSOG in vitro depends on the intracellular localization, and the cytotoxic effect is 48% for the cytoplasmic localization of the fusion protein, 65% for the mitochondrial localization, and 69% for the membrane localization. The obtained data indicate that, for maximization of the photodynamic effect in vivo, it is appropriate to use the NanoLuc–miniSOG fusion protein in the membrane localization.

Язык оригиналаАнглийский
Страницы (с-по)228-230
Число страниц3
ЖурналDoklady Biochemistry and Biophysics
Том474
Номер выпуска1
DOI
СостояниеОпубликовано - 1 мая 2017
Опубликовано для внешнего пользованияДа

ASJC Scopus subject areas

  • Biophysics
  • Chemistry(all)
  • Medicine(all)
  • Biochemistry

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