Expression of the chimeric IgE gene in cell culture and in various mouse tissues

D. V. Rodin, B. V. Rad'ko, V. A. Kolesnikov, O. L. Polanovsky, S. M. Deyev

Результат исследований: Материалы для журналаСтатья

6 Цитирования (Scopus)

Выдержка

Transient expression of recombinant gene constructs is now more widely used in gene therapy as well as in DNA vaccination. In this study, the ability of one and the same genetic construct to drive gene expression both in cell culture and in tissues of the whole organism was demonstrated. Chinese hamster ovarian cells (CHO) were transfected in vitro with plasmids bearing the genes for chimeric IgE (mouse/human) antibodies under control of the human cytomegalovirus (hCMV) promoter. Secretion of recombinant IgE antibodies by transfected cells reached 60% of the intracellular concentration of antibodies. The same gene constructs were introduced into various mouse tissues using ballistic transfection in vivo. The IgE content in blood after transfection of cartilage was found to be several times lower than after transfection of the liver, spleen, or foot pad. At the same time, the content of antibodies to the xenogenous determinants of IgE was essentially independent of the tissue type. These data can be employed in selecting conditions for genetic immunization and gene therapy. © 2004 Elsevier SAS. All rights reserved.
Язык оригиналаАнглийский
Страницы (с-по)939-943
Число страниц5
ЖурналBiochimie
Том86
Номер выпуска12
DOI
СостояниеОпубликовано - 1 дек 2004
Опубликовано для внешнего пользованияДа

Отпечаток

Cell culture
Immunoglobulin E
Cell Culture Techniques
Genes
Genetic Therapy
Tissue
Transfection
Gene therapy
Antibodies
Bearings (structural)
Immunization
Gene Expression
Cartilage
Ballistics
Cricetulus
Cytomegalovirus
Gene expression
Liver
Foot
Vaccination

Цитировать

Expression of the chimeric IgE gene in cell culture and in various mouse tissues. / Rodin, D. V.; Rad'ko, B. V.; Kolesnikov, V. A.; Polanovsky, O. L.; Deyev, S. M.

В: Biochimie, Том 86, № 12, 01.12.2004, стр. 939-943.

Результат исследований: Материалы для журналаСтатья

Rodin, DV, Rad'ko, BV, Kolesnikov, VA, Polanovsky, OL & Deyev, SM 2004, 'Expression of the chimeric IgE gene in cell culture and in various mouse tissues', Biochimie, том. 86, № 12, стр. 939-943. https://doi.org/10.1016/j.biochi.2004.07.016
Rodin, D. V. ; Rad'ko, B. V. ; Kolesnikov, V. A. ; Polanovsky, O. L. ; Deyev, S. M. / Expression of the chimeric IgE gene in cell culture and in various mouse tissues. В: Biochimie. 2004 ; Том 86, № 12. стр. 939-943.
@article{39fde5f6cf9140eba732adaad3da1119,
title = "Expression of the chimeric IgE gene in cell culture and in various mouse tissues",
abstract = "Transient expression of recombinant gene constructs is now more widely used in gene therapy as well as in DNA vaccination. In this study, the ability of one and the same genetic construct to drive gene expression both in cell culture and in tissues of the whole organism was demonstrated. Chinese hamster ovarian cells (CHO) were transfected in vitro with plasmids bearing the genes for chimeric IgE (mouse/human) antibodies under control of the human cytomegalovirus (hCMV) promoter. Secretion of recombinant IgE antibodies by transfected cells reached 60{\%} of the intracellular concentration of antibodies. The same gene constructs were introduced into various mouse tissues using ballistic transfection in vivo. The IgE content in blood after transfection of cartilage was found to be several times lower than after transfection of the liver, spleen, or foot pad. At the same time, the content of antibodies to the xenogenous determinants of IgE was essentially independent of the tissue type. These data can be employed in selecting conditions for genetic immunization and gene therapy. {\circledC} 2004 Elsevier SAS. All rights reserved.",
keywords = "Gene expression, Genes of chimeric (mouse/man) antibodies, Immunoglobulin E, Transfection",
author = "Rodin, {D. V.} and Rad'ko, {B. V.} and Kolesnikov, {V. A.} and Polanovsky, {O. L.} and Deyev, {S. M.}",
year = "2004",
month = "12",
day = "1",
doi = "10.1016/j.biochi.2004.07.016",
language = "English",
volume = "86",
pages = "939--943",
journal = "Biochimie",
issn = "0300-9084",
publisher = "Elsevier",
number = "12",

}

TY - JOUR

T1 - Expression of the chimeric IgE gene in cell culture and in various mouse tissues

AU - Rodin, D. V.

AU - Rad'ko, B. V.

AU - Kolesnikov, V. A.

AU - Polanovsky, O. L.

AU - Deyev, S. M.

PY - 2004/12/1

Y1 - 2004/12/1

N2 - Transient expression of recombinant gene constructs is now more widely used in gene therapy as well as in DNA vaccination. In this study, the ability of one and the same genetic construct to drive gene expression both in cell culture and in tissues of the whole organism was demonstrated. Chinese hamster ovarian cells (CHO) were transfected in vitro with plasmids bearing the genes for chimeric IgE (mouse/human) antibodies under control of the human cytomegalovirus (hCMV) promoter. Secretion of recombinant IgE antibodies by transfected cells reached 60% of the intracellular concentration of antibodies. The same gene constructs were introduced into various mouse tissues using ballistic transfection in vivo. The IgE content in blood after transfection of cartilage was found to be several times lower than after transfection of the liver, spleen, or foot pad. At the same time, the content of antibodies to the xenogenous determinants of IgE was essentially independent of the tissue type. These data can be employed in selecting conditions for genetic immunization and gene therapy. © 2004 Elsevier SAS. All rights reserved.

AB - Transient expression of recombinant gene constructs is now more widely used in gene therapy as well as in DNA vaccination. In this study, the ability of one and the same genetic construct to drive gene expression both in cell culture and in tissues of the whole organism was demonstrated. Chinese hamster ovarian cells (CHO) were transfected in vitro with plasmids bearing the genes for chimeric IgE (mouse/human) antibodies under control of the human cytomegalovirus (hCMV) promoter. Secretion of recombinant IgE antibodies by transfected cells reached 60% of the intracellular concentration of antibodies. The same gene constructs were introduced into various mouse tissues using ballistic transfection in vivo. The IgE content in blood after transfection of cartilage was found to be several times lower than after transfection of the liver, spleen, or foot pad. At the same time, the content of antibodies to the xenogenous determinants of IgE was essentially independent of the tissue type. These data can be employed in selecting conditions for genetic immunization and gene therapy. © 2004 Elsevier SAS. All rights reserved.

KW - Gene expression

KW - Genes of chimeric (mouse/man) antibodies

KW - Immunoglobulin E

KW - Transfection

UR - http://www.scopus.com/inward/record.url?scp=12744268201&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=12744268201&partnerID=8YFLogxK

U2 - 10.1016/j.biochi.2004.07.016

DO - 10.1016/j.biochi.2004.07.016

M3 - Article

C2 - 15667945

AN - SCOPUS:12744268201

VL - 86

SP - 939

EP - 943

JO - Biochimie

JF - Biochimie

SN - 0300-9084

IS - 12

ER -