Expression of humanized anti-Her2/neu single-chain IgG1-like antibody in mammary glands of transgenic mice

Veronika Yuskevich, Yuriy Khodarovich, German Kagarliskiy, Oleg Stremovskiy, Oksana Maksimenko, Sergey Lukash, Oleg Polanovsky, Sergey Deyev

Результат исследований: Материалы для журналаСтатья

6 Цитирования (Scopus)

Выдержка

A system for production of single-chain antibody in mammary glands of mice was developed on the basis of a hybrid gene constructed from the coding sequence of anti-Her2/neu single-chain antibody inserted into the first exon of the sheep beta-lactoglobulin gene. Lines of transgenic mice were obtained that expressed humanized single-chain anti-Her2/neu IgG1-like antibody in their milk. These antibodies interact with Her2/neu antigen with high affinity (K d = 0.4 nM). The expression level of the transgene depended on its integration site in the genome but not on the copy number. The transgene had no toxic effect on the mice and was stably inherited, at least for two generations. The results reveal new opportunities of producing single-chain antibodies in the milk of animals.

Язык оригиналаАнглийский
Страницы (с-по)628-630
Число страниц3
ЖурналBiochimie
Том93
Номер выпуска3
DOI
СостояниеОпубликовано - мар 2011
Опубликовано для внешнего пользованияДа

Отпечаток

Single-Chain Antibodies
Human Mammary Glands
Transgenic Mice
Immunoglobulin G
Genes
Transgenes
Antibodies
Milk
Lactoglobulins
Poisons
Exons
Sheep
Animals
Genome
Antigens

ASJC Scopus subject areas

  • Biochemistry

Цитировать

Yuskevich, V., Khodarovich, Y., Kagarliskiy, G., Stremovskiy, O., Maksimenko, O., Lukash, S., ... Deyev, S. (2011). Expression of humanized anti-Her2/neu single-chain IgG1-like antibody in mammary glands of transgenic mice. Biochimie, 93(3), 628-630. https://doi.org/10.1016/j.biochi.2010.12.001

Expression of humanized anti-Her2/neu single-chain IgG1-like antibody in mammary glands of transgenic mice. / Yuskevich, Veronika; Khodarovich, Yuriy; Kagarliskiy, German; Stremovskiy, Oleg; Maksimenko, Oksana; Lukash, Sergey; Polanovsky, Oleg; Deyev, Sergey.

В: Biochimie, Том 93, № 3, 03.2011, стр. 628-630.

Результат исследований: Материалы для журналаСтатья

Yuskevich, V, Khodarovich, Y, Kagarliskiy, G, Stremovskiy, O, Maksimenko, O, Lukash, S, Polanovsky, O & Deyev, S 2011, 'Expression of humanized anti-Her2/neu single-chain IgG1-like antibody in mammary glands of transgenic mice', Biochimie, том. 93, № 3, стр. 628-630. https://doi.org/10.1016/j.biochi.2010.12.001
Yuskevich V, Khodarovich Y, Kagarliskiy G, Stremovskiy O, Maksimenko O, Lukash S и соавт. Expression of humanized anti-Her2/neu single-chain IgG1-like antibody in mammary glands of transgenic mice. Biochimie. 2011 Март;93(3):628-630. https://doi.org/10.1016/j.biochi.2010.12.001
Yuskevich, Veronika ; Khodarovich, Yuriy ; Kagarliskiy, German ; Stremovskiy, Oleg ; Maksimenko, Oksana ; Lukash, Sergey ; Polanovsky, Oleg ; Deyev, Sergey. / Expression of humanized anti-Her2/neu single-chain IgG1-like antibody in mammary glands of transgenic mice. В: Biochimie. 2011 ; Том 93, № 3. стр. 628-630.
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AU - Stremovskiy, Oleg

AU - Maksimenko, Oksana

AU - Lukash, Sergey

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