TY - JOUR
T1 - Electrochemical nonenzymatic sensor for cholesterol determination in food
AU - Derina, Ksenia
AU - Korotkova, Elena
AU - Taishibekova, Yekaterina
AU - Salkeeva, Lyazat
AU - Kratochvil, Bohumil
AU - Barek, Jiri
PY - 2018/6/16
Y1 - 2018/6/16
N2 - The treatment of some inborn metabolism errors requires cholesterol substitution therapy. Cholesterol plays a vital role in the human body. Therefore, the majority of cholesterol determination techniques are targeted to blood and blood serum. Nevertheless, cholesterol determination in food is important as well. In this paper, cholesterol determination using differential pulse voltammetry (DPV) in dairy products (e.g., milk, clotted cream, yogurt, butter, etc.) is reported with a novel nonenzymatic sensor based on diphosphonic acid of 1,4-diacetylglycoluril (DPADGU) as an electrode surface modifier. Stable anodic response was obtained from cholesterol on the modified carbon-based electrode. The sensor has high stability, sensitivity (20 μA mol L−1 cm−2), and a wide linear range from 1 up to 200 μM. The LOD and LOQ values are 1.5 and 5.1 μM, respectively. The developed methods were successfully applied to the above mentioned dairy products. [Figure not available: see fulltext.]
AB - The treatment of some inborn metabolism errors requires cholesterol substitution therapy. Cholesterol plays a vital role in the human body. Therefore, the majority of cholesterol determination techniques are targeted to blood and blood serum. Nevertheless, cholesterol determination in food is important as well. In this paper, cholesterol determination using differential pulse voltammetry (DPV) in dairy products (e.g., milk, clotted cream, yogurt, butter, etc.) is reported with a novel nonenzymatic sensor based on diphosphonic acid of 1,4-diacetylglycoluril (DPADGU) as an electrode surface modifier. Stable anodic response was obtained from cholesterol on the modified carbon-based electrode. The sensor has high stability, sensitivity (20 μA mol L−1 cm−2), and a wide linear range from 1 up to 200 μM. The LOD and LOQ values are 1.5 and 5.1 μM, respectively. The developed methods were successfully applied to the above mentioned dairy products. [Figure not available: see fulltext.]
KW - Cholesterol
KW - Dairy products
KW - Sensor
KW - Smith-Lemli-Opitz syndrome
KW - Voltammetry
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U2 - 10.1007/s00216-018-1164-x
DO - 10.1007/s00216-018-1164-x
M3 - Article
AN - SCOPUS:85048583615
SP - 1
EP - 8
JO - Fresenius Zeitschrift fur Analytische Chemie
JF - Fresenius Zeitschrift fur Analytische Chemie
SN - 0016-1152
ER -