Efficiency of Bioluminescence Resonance Energy Transfer in the NanoLuc-miniSOG-Furimazine System

E. I. Shramova, S. M. Deyev, G. M. Proshkina

Результат исследований: Материалы для журналаСтатьярецензирование

2 Цитирования (Scopus)

Аннотация

Photodynamic therapy (PDT) is a clinically approved, minimally invasive method for tumor destruction in the presence of a photosensitizer (PS), oxygen, and a light source. The main obstacle for the PDT treatment of deep tumors is the problem of the excitation light delivery inside the body without reduction of light intensity. An internal light source based on a bioluminescence system NanoLuc-furimazine can be considered as an alternative to an external light source. This system can be successfully used to excite a protein photosensitizer miniSOG and induce its phototoxicity in cancer cells as a result of bioluminescent resonance energy transfer. In this paper, the effectiveness of bioluminescent resonance energy transfer in the NanoLuc-miniSOG-furimazine system in living cells was studied. The efficiency of the energy transfer in this system was found to be independent of the furimazine concentration, while intracellular localization of the NanoLuc-miniSOG hybrid protein was found to be a crucial factor.

Язык оригиналаАнглийский
Страницы (с-по)755-758
Число страниц4
ЖурналRussian Journal of Bioorganic Chemistry
Том44
Номер выпуска6
DOI
СостояниеОпубликовано - 1 ноя 2018
Опубликовано для внешнего пользованияДа

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry

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