Effect of nanosecond HPM pulses on individual development of Drosophilas: Estimation of comparative contribution by microwave and X-ray components

Effect of X-band HPM pulses from SINUS-500 relativistic HPM source on Drosophila melanogaster embryos, larvae, and pupae was studied. The test objects were irradiated with 10-ns pulses during 5-min batches at pulse rate of 3-29 p.p.s. The peak microwave power was 500 MW, the electric field in the irradiation zone was ∼1.5×10⁴ V/cm and the X-ray dose was 1-3 μRad per pulse. To understand the mechanisms of the effects and to estimate the comparative contribution of microwave and X-ray components of the SINUS-500 radiation, the objects were also exposed to (a) X-ray-free microwave pulses from a magnetron (wavelength 3 cm, pulse width 730 ns, pulse power up to 40 kW, electric field at the object ∼1.5×10³ V/cm), (b) pure X-ray pulses produced in periodically-pulsed regime by the SINUS-500 facility where the microwave component was cut by a metal screen, and (c) continuous X-rays with nearly the same dose and exposition time (2-30 mRad during 5 min). The irradiation caused notable disturbance in individual development of Drosophilas in the form of increased PID, teratism of imagoes, delay of imago fly etc. The strength of the effects was depending upon the stage of development of the organism by the moment of irradiation, and the pulse repetition rate. Repeated extra exposures did not increase the effect. The pulses from SINUS-500 facility produced stronger effect that the magnetron pulses. The strength of X-ray pulse action depended on the pulse rate while the effect from continuous X-rays was negligibly small. The paper discusses, possible mechanisms of action of nanosecond HPM pulses.