HPLC systems with electrochemical, UV spectrophotometric, and fluorescent detectors were used to determine genotoxic derivatives of biphenyl - 4-aminobiphenyl (4-ABP) and 4-nitrobiphenyl (4-NBP). As 4-NBP cannot be determined by electrochemical and fluorescent detector directly, reduction of 4-NBP to 4-ABP (making use of a titanium(III)-based reducing agent) was investigated and optimized. UV detection was found to be the least sensitive and fluorescent detection the most sensitive one. Limits of detection (LDs) for 4-ABP in the presence of a reducing agent were 6.0 × 10-6 mol L-1, 2.0 × 10-6 mol L-1, and 8.0 × 10-8 mol L-1for UV, electrochemical, and fluorescent detection, respectively. For the determination of 4-NBP after its reduction to 4-ABP, the obtained LDs were 8.0 × 10-6 mol L-1 (UV), 4.0 × 10-6 mol L-1 (electrochemical), and 2.0 × 10-7 mol L-1 (fluorescent detection). Moreover, it wasalso possible to determine 4-NBP directly using HPLC with UV detection, where the LD was 4.0 × 10-7 molL-1.
|Журнал||International Journal of Electrochemical Science|
|Состояние||Опубликовано - 1 фев 2013|
|Опубликовано для внешнего пользования||Да|
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