One of the current areas of medical materials science is the formation of ultrathin coatings on the surface of implants. Among the many types of coating used in vascular surgery, binary and ternary titanium compounds have good prospects due to their high bio- and hemocompatibility. The properties of the coating based on films of nitrogen-containing titanium oxide (titanium oxynitride), applied by reactive magnetron sputtering (RMS), was studied. While problems of establishing and maintaining the necessary spraying mode is relevant for the RMS technique. The cytotoxicity of the titanium oxy nitride compounds obtained under various modes of functioning of RMS on endothelial cells line EA.hy 926 was the aim of this report. It was showed that the levels of nitric oxide (NO) production by EA.hy 926 cell line on Day 1 and Day 5 growth under the different samples of nitinol treated with different RMS modes was comparable to control. It was no obtained cytotoxic effect of the nitrogen-containing coatings of titanium oxide obtained by various modes of RMS on EA.hy 926 cell line. While was observed reduced levels of NO production by EA.hy 926 cell line on Day 3.