Continuous Normobaric Hypoxia Improved Cardiac Bioenergetics after Ischemia/Reperfusion: Role of Opioid Receptors

E. S. Prokudina, N. V. Naryzhnaya, E. A. Nesterov, S. Yu Tsibulnikov, L. N. Maslov

Результат исследований: Материалы для журналаСтатья

Аннотация

We analyzed the role of opioid receptors in the conditioning effect of continuous normobaric hypoxia on bioenergetics of the heart subjected to ischemia/reperfusion injury. Male Wistar rats were adapted to a 21-day continuous normobaric hypoxia (12% pO2). Then, the hearts were isolated and subjected to 45-min total ischemia followed by 30-min reperfusion. Damage to the myocardium was assessed by activity of creatine phosphokinase in the perfusate. Experiments on isolated mitochondria showed that ischemia/reperfusion injury decreased the respiration rate in state 3 (V3), the ratio of added ADP and oxygen consumption in respiration state 3 (ADP/O ratio), the mitochondrial potential across the inner membrane (Δψ), and Ca2+ binding capacity of mitochondria. In addition, ischemia/reperfusion injury decreased myocardial ATP. Preventive continuous normobaric hypoxia pronouncedly moderated these adverse effects of reperfusion. It was found that its protective effects were related to activation of cardiac μ- and δ2-opioid receptors.

Язык оригиналаАнглийский
Страницы (с-по)13-17
Число страниц5
ЖурналBulletin of Experimental Biology and Medicine
Том169
Номер выпуска1
DOI
СостояниеОпубликовано - 1 мая 2020

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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