TY - JOUR
T1 - Composite ferroelectric membranes based on vinylidene fluoride-tetrafluoroethylene copolymer and polyvinylpyrrolidone for wound healing
AU - Tverdokhlebova, Tamara S.
AU - Antipina, Ludmila S.
AU - Kudryavtseva, Valeriya L.
AU - Stankevich, Ksenia S.
AU - Kolesnik, Ilya M.
AU - Senokosova, Evgenia A.
AU - Velikanova, Elena A.
AU - Antonova, Larisa V.
AU - Vasilchenko, Dmitry V.
AU - Dambaev, Georgiy T.
AU - Plotnikov, Evgenii V.
AU - Bouznik, Vyacheslav M.
AU - Bolbasov, Evgeny N.
N1 - Funding Information:
Funding: Membranes fabrication, physicochemical properties and in vivo studies were funded by RFBR, project number # 20-03-00171. In vitro studies was supported by the Complex Program of Basic Research under the Siberian Branch of the Russian Academy of Sciences within the Basic Research Topic of Research Institute for Complex Issues of Cardiovascular Diseases # 0546-2019-0002 “Pathogenetic basis for the development of cardiovascular implants from biocompatible materials using patient-oriented approach, mathematical modeling, tissue engineering, and genomic predictors”.
Publisher Copyright:
© 2020 by the authors.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2021/1
Y1 - 2021/1
N2 - Wound healing is a complex process and an ongoing challenge for modern medicine. Herein, we present the results of study of structure and properties of ferroelectric composite polymer membranes for wound healing. Membranes were fabricated by electrospinning from a solution of vinylidene fluoride/tetrafluoroethylene copolymer (VDF–TeFE) and polyvinylpyrrolidone (PVP) in dimethylformamide (DMF). The effects of the PVP content on the viscosity and conductivity of the spinning solution, DMF concentration, chemical composition, crystal structure, and conformation of VDF–TeFE macromolecules in the fabricated materials were studied. It was found that as PVP amount increased, the viscosity and conductivity of the spinning solutions decreased, resulting in thinner fibers. Using FTIR and XRD methods, it was shown that if the PVP content was lower than 50 wt %, the VDF–TeFE copolymer adopted a flat zigzag conformation (TTT conformation) and crystalline phases with ferroelectric properties were formed. Gas chromatography results indicated that an increase in the PVP concentration led to a higher residual amount of DMF in the material, causing cytotoxic effects on 3T3L1 fibroblasts. In vivo studies demonstrated that compared to classical gauze dressings impregnated with a solution of an antibacterial agent, ferroelectric composite membranes with 15 wt % PVP provided better conditions for the healing of purulent wounds.
AB - Wound healing is a complex process and an ongoing challenge for modern medicine. Herein, we present the results of study of structure and properties of ferroelectric composite polymer membranes for wound healing. Membranes were fabricated by electrospinning from a solution of vinylidene fluoride/tetrafluoroethylene copolymer (VDF–TeFE) and polyvinylpyrrolidone (PVP) in dimethylformamide (DMF). The effects of the PVP content on the viscosity and conductivity of the spinning solution, DMF concentration, chemical composition, crystal structure, and conformation of VDF–TeFE macromolecules in the fabricated materials were studied. It was found that as PVP amount increased, the viscosity and conductivity of the spinning solutions decreased, resulting in thinner fibers. Using FTIR and XRD methods, it was shown that if the PVP content was lower than 50 wt %, the VDF–TeFE copolymer adopted a flat zigzag conformation (TTT conformation) and crystalline phases with ferroelectric properties were formed. Gas chromatography results indicated that an increase in the PVP concentration led to a higher residual amount of DMF in the material, causing cytotoxic effects on 3T3L1 fibroblasts. In vivo studies demonstrated that compared to classical gauze dressings impregnated with a solution of an antibacterial agent, ferroelectric composite membranes with 15 wt % PVP provided better conditions for the healing of purulent wounds.
KW - Composite
KW - Ferroelectrics
KW - Membranes
KW - Polyvinylpyrrolidone
KW - Wound healing
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U2 - 10.3390/membranes11010021
DO - 10.3390/membranes11010021
M3 - Article
AN - SCOPUS:85098881838
VL - 11
SP - 1
EP - 16
JO - Membranes
JF - Membranes
SN - 2077-0375
IS - 1
M1 - 21
ER -