Выдержка
An innovative microcarrier based on a carboxy-enriched and branched polylactic acid derivative was developed to enhance the in vitro phototoxicity of the photosensitizer and prodrug 5-aminolevulinic. Microparticles, prepared by double emulsion technique and loaded with the prodrug were carefully characterized and the effect of the polymer structure on the chemical, physical and biological properties of the final product was evaluated. Results showed that microparticles have a spherical shape and ability to allocate up to 30 μg of the photosensitizer per mg of carrier despite their difference in solubility. Release studies performed in various simulated physiological conditions demonstrate the influence of the branched structure and the presence of the additional carboxylic groups on the release rate and the possibility to modulate it. In vitro assays conducted on human epithelial adenocarcinoma cells proved the not cytotoxicity of the carriers in a wide range of concentrations. The hemocompatibility and surface proteins adsorption were evaluated at different microparticles concentrations to evaluate the safety and estimate the possible microparticles residential time in the bloodstream. The advantages, of loading 5-aminolevulinic acid in the prepared carrier has been deeply described in terms of enhanced phototoxicity, compared to the free 5-aminolevulinic acid formulation after irradiation with light at 635 nm. The obtained results demonstrate the advantages of the prepared derivative compared to the linear polylactide for future application in photodynamic therapy based on the photosensitizer 5-aminolevulinic acid.
| Язык оригинала | Английский |
|---|---|
| Страницы (с-по) | 80-88 |
| Число страниц | 9 |
| Журнал | Journal of Photochemistry and Photobiology B: Biology |
| Том | 181 |
| DOI | |
| Состояние | Опубликовано - 1 апр 2018 |
Отпечаток
ASJC Scopus subject areas
- Radiation
- Radiological and Ultrasound Technology
- Biophysics
- Radiology Nuclear Medicine and imaging
Цитировать
Branched poly (lactic acid) microparticles for enhancing the 5-aminolevulinic acid phototoxicity. / Di Martino, Antonio; Trusova, Marina E.; Postnikov, Pavel S.; Sedlarik, Vladimir.
В: Journal of Photochemistry and Photobiology B: Biology, Том 181, 01.04.2018, стр. 80-88.Результат исследований: Материалы для журнала › Статья
}
TY - JOUR
T1 - Branched poly (lactic acid) microparticles for enhancing the 5-aminolevulinic acid phototoxicity
AU - Di Martino, Antonio
AU - Trusova, Marina E.
AU - Postnikov, Pavel S.
AU - Sedlarik, Vladimir
PY - 2018/4/1
Y1 - 2018/4/1
N2 - An innovative microcarrier based on a carboxy-enriched and branched polylactic acid derivative was developed to enhance the in vitro phototoxicity of the photosensitizer and prodrug 5-aminolevulinic. Microparticles, prepared by double emulsion technique and loaded with the prodrug were carefully characterized and the effect of the polymer structure on the chemical, physical and biological properties of the final product was evaluated. Results showed that microparticles have a spherical shape and ability to allocate up to 30 μg of the photosensitizer per mg of carrier despite their difference in solubility. Release studies performed in various simulated physiological conditions demonstrate the influence of the branched structure and the presence of the additional carboxylic groups on the release rate and the possibility to modulate it. In vitro assays conducted on human epithelial adenocarcinoma cells proved the not cytotoxicity of the carriers in a wide range of concentrations. The hemocompatibility and surface proteins adsorption were evaluated at different microparticles concentrations to evaluate the safety and estimate the possible microparticles residential time in the bloodstream. The advantages, of loading 5-aminolevulinic acid in the prepared carrier has been deeply described in terms of enhanced phototoxicity, compared to the free 5-aminolevulinic acid formulation after irradiation with light at 635 nm. The obtained results demonstrate the advantages of the prepared derivative compared to the linear polylactide for future application in photodynamic therapy based on the photosensitizer 5-aminolevulinic acid.
AB - An innovative microcarrier based on a carboxy-enriched and branched polylactic acid derivative was developed to enhance the in vitro phototoxicity of the photosensitizer and prodrug 5-aminolevulinic. Microparticles, prepared by double emulsion technique and loaded with the prodrug were carefully characterized and the effect of the polymer structure on the chemical, physical and biological properties of the final product was evaluated. Results showed that microparticles have a spherical shape and ability to allocate up to 30 μg of the photosensitizer per mg of carrier despite their difference in solubility. Release studies performed in various simulated physiological conditions demonstrate the influence of the branched structure and the presence of the additional carboxylic groups on the release rate and the possibility to modulate it. In vitro assays conducted on human epithelial adenocarcinoma cells proved the not cytotoxicity of the carriers in a wide range of concentrations. The hemocompatibility and surface proteins adsorption were evaluated at different microparticles concentrations to evaluate the safety and estimate the possible microparticles residential time in the bloodstream. The advantages, of loading 5-aminolevulinic acid in the prepared carrier has been deeply described in terms of enhanced phototoxicity, compared to the free 5-aminolevulinic acid formulation after irradiation with light at 635 nm. The obtained results demonstrate the advantages of the prepared derivative compared to the linear polylactide for future application in photodynamic therapy based on the photosensitizer 5-aminolevulinic acid.
KW - 5-Aminolevulinic acid
KW - Drug delivery
KW - Microparticles
KW - Photodynamic therapy
KW - Polylactic acid
UR - http://www.scopus.com/inward/record.url?scp=85042943026&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85042943026&partnerID=8YFLogxK
U2 - 10.1016/j.jphotobiol.2018.03.001
DO - 10.1016/j.jphotobiol.2018.03.001
M3 - Article
AN - SCOPUS:85042943026
VL - 181
SP - 80
EP - 88
JO - Journal of Photochemistry and Photobiology B: Biology
JF - Journal of Photochemistry and Photobiology B: Biology
SN - 1011-1344
ER -