Bone-like multilevel calcium phosphate coating modulates an interaction of mesenchymal stem cells and tumor cells

V. V. Malashchenko, E. O. Shunkin, V. V. Shupletsova, O. G. Khaziakhmatova, K. A. Yurova, E. S. Melashchenko, L. S. Litvinova, I. A. Khlusov, E. G. Komarova, V. V. Chebodaeva, Yu P. Sharkeev

Результат исследований: Материалы для книги/типы отчетовМатериалы для конференции

Аннотация

Modern biomaterial biocompatibility research focuses the biomaterial hierarchic effects on multipotent mesenchymal stromal cell (MMSC) behavior (that occur at the nano-, micro- and macroscales) because MMSCs are the fundamental units that produce/regenerate bone tissue. Leukemia initiation and progression are connected with a disfunction of health cell microenvironment and MMSCs. Continuous monitoring of MMSC and tumor cell interaction is a promising tool for oncology, cellular biology, biotechnology and environmental research. The aim was to investigate a modulation of in vitro interaction of human MMSCs and leukemic T lymphoblast-like cells (Jurkat T cells) caused by micro-arc multilevel calcium phosphate (CP) coating with the help of Cell-IQ and RTCA advanced tools for continuous monitoring. An average velocity of cell division (AVCD) of human adipose-derived MMSCs (hAMMSCs) contacted in vitro with allogenic Jurkat line of human leukemic T lymphoblasts (Jurkat T cells) was studied by means of Cell-IQ v2 MLF integrated phase-contrast microscopic platform for real-time surveillance imaging of living cells. Both 50 μL suspensions (5×104 viable karyocytes) of the CD73CD90CD105+ adherent cells and Jurkat T cells were applied into the center of the well of 12-well plastic plates for 7 days at 100% humidity in a 5% CO2 atmosphere at 37°C until a monolayer formation. A nutrient medium was once replaced. To determine cell invasion (chemotactic motility) through 8 μm pores the real-time cell analysis (RTCA DP Analyzer) with the CIM-plate was used. AVCD of fibroblast-like adherent hAMMSCs was 0.27-0.63 divisions/h. CP coating diminished significantly the percent of dividing hAMMSCs contacted with leukemic Jurkat T cells. RTCA system showed significant hAMMSC invasion towards tumor cells and not vice versa. For all this, cellular interaction led to increasing viability of Jurkat T cells and decreasing hAMMSC viability. Thus, tumor Jurkat T cells could control a fate of health hAMMSCs and promote stromal microenvironment for survivability of tumor clones by means of secretable molecular products. Multilevel micro-arc CP coating forms bone-like inorganic structure that is capable to modulate in vitro interaction of human MMSCs and leukemic T lymphoblasts. The results obtained may be useful for replacement surgery applications of orthopedic implants in cancer patients.

Язык оригиналаАнглийский
Название основной публикацииProceedings of the International Conference on Advanced Materials with Hierarchical Structure for New Technologies and Reliable Structures 2019
РедакторыVictor E. Panin, Sergey G. Psakhie, Vasily M. Fomin
ИздательAmerican Institute of Physics Inc.
ISBN (электронное издание)9780735419124
DOI
СостояниеОпубликовано - 19 ноя 2019
СобытиеInternational Conference on Advanced Materials with Hierarchical Structure for New Technologies and Reliable Structures 2019 - Tomsk, Российская Федерация
Продолжительность: 1 окт 20195 окт 2019

Серия публикаций

НазваниеAIP Conference Proceedings
Том2167
ISSN (печатное издание)0094-243X
ISSN (электронное издание)1551-7616

Конференция

КонференцияInternational Conference on Advanced Materials with Hierarchical Structure for New Technologies and Reliable Structures 2019
СтранаРоссийская Федерация
ГородTomsk
Период1.10.195.10.19

ASJC Scopus subject areas

  • Physics and Astronomy(all)

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  • Цитировать

    Malashchenko, V. V., Shunkin, E. O., Shupletsova, V. V., Khaziakhmatova, O. G., Yurova, K. A., Melashchenko, E. S., Litvinova, L. S., Khlusov, I. A., Komarova, E. G., Chebodaeva, V. V., & Sharkeev, Y. P. (2019). Bone-like multilevel calcium phosphate coating modulates an interaction of mesenchymal stem cells and tumor cells. В V. E. Panin, S. G. Psakhie, & V. M. Fomin (Ред.), Proceedings of the International Conference on Advanced Materials with Hierarchical Structure for New Technologies and Reliable Structures 2019 [020215] (AIP Conference Proceedings; Том 2167). American Institute of Physics Inc.. https://doi.org/10.1063/1.5132082