Biosynthesis of the scFv Antibody to Human Ferritin in Plant and Bacterial Producers

E. G. Semenyuk, O. A. Stremovskii, I. V. Orlova, T. G. Balandin, A. M. Nosov, Ya I. Buryanov, S. M. Deyev, R. V. Petrov

Результат исследований: Материалы для журналаСтатья

3 Цитирования (Scopus)

Аннотация

A recombinant scFv antibody against human spleen ferritin was expressed as a barstar-fused protein in Escherichia coli and in Nicotiana tabacum plants and suspension cell cultures. As demonstrated by immunoblotting with antibarstar antibodies, direction of the recombinant protein to the endomembrane system of plant cells ensured its stability and solubility. Production of the recombinant protein did not differ between parental transgenic plants and their first-generation progeny. Fusion with barstar allowed not only immunochemical detection of the recombinant scFv antibody, but also their purification from the plant material by affinity chromatography with barnase-His6 immobilized on a metal-affinity carrier.

Язык оригиналаАнглийский
Страницы (с-по)780-786
Число страниц7
ЖурналMolecular Biology
Том37
Номер выпуска5
DOI
СостояниеОпубликовано - сен 2003
Опубликовано для внешнего пользованияДа

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics

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  • Цитировать

    Semenyuk, E. G., Stremovskii, O. A., Orlova, I. V., Balandin, T. G., Nosov, A. M., Buryanov, Y. I., Deyev, S. M., & Petrov, R. V. (2003). Biosynthesis of the scFv Antibody to Human Ferritin in Plant and Bacterial Producers. Molecular Biology, 37(5), 780-786. https://doi.org/10.1023/A:1026005614970