TY - JOUR
T1 - Bioluminescence as a tool for studying detoxification processes in metal salt solutions involving humic substances
AU - Tarasova, A. S.
AU - Kislan, S. L.
AU - Fedorova, E. S.
AU - Kuznetsov, A. M.
AU - Mogilnaya, O. A.
AU - Stom, D. I.
AU - Kudryasheva, N. S.
PY - 2012/12/5
Y1 - 2012/12/5
N2 - The paper considers effects of humic substances (HS), as natural attenuators of toxicity, on solutions of model inorganic pollutants, metal salts - Pb(NO3)2, cl2, CuSO4, Eu(NO 3)3, cl3, and K3[Fe(N)6]. Luminous bacteria Photobacterium phosphoreum and bioluminescent system of coupled enzymatic reactions were used as bioassays to monitor toxicity of salt solutions. The ability of HS to decrease or increase toxicity was demonstrated. Detoxifying concentrations of HS were determined; detoxification coefficients were calculated at different times of exposure of salt solutions to HS. To study the combined effects of HS and salts on bioluminescent assay systems, the rates of biochemical reactions and bacterial ultrastructure were analyzed. The detoxifying effects were explained by: (1) decrease of free metal content in water solutions under metal-HS binding; (2) increase of biochemical reaction rates in a bioluminescent assay system under HS effect; (3) enhancement of mucous layers on cell surface as a response to unfavorable impact of toxicants. Detoxifying mechanisms (2) and (3) reveal the active role of bioassay systems in detoxification processes.
AB - The paper considers effects of humic substances (HS), as natural attenuators of toxicity, on solutions of model inorganic pollutants, metal salts - Pb(NO3)2, cl2, CuSO4, Eu(NO 3)3, cl3, and K3[Fe(N)6]. Luminous bacteria Photobacterium phosphoreum and bioluminescent system of coupled enzymatic reactions were used as bioassays to monitor toxicity of salt solutions. The ability of HS to decrease or increase toxicity was demonstrated. Detoxifying concentrations of HS were determined; detoxification coefficients were calculated at different times of exposure of salt solutions to HS. To study the combined effects of HS and salts on bioluminescent assay systems, the rates of biochemical reactions and bacterial ultrastructure were analyzed. The detoxifying effects were explained by: (1) decrease of free metal content in water solutions under metal-HS binding; (2) increase of biochemical reaction rates in a bioluminescent assay system under HS effect; (3) enhancement of mucous layers on cell surface as a response to unfavorable impact of toxicants. Detoxifying mechanisms (2) and (3) reveal the active role of bioassay systems in detoxification processes.
KW - Bioluminescent assay
KW - Enzyme reaction rates
KW - Humic substances
KW - Metal salts
KW - Photobacterium phosphoreum
KW - Toxicity
UR - http://www.scopus.com/inward/record.url?scp=84868244356&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84868244356&partnerID=8YFLogxK
U2 - 10.1016/j.jphotobiol.2012.09.020
DO - 10.1016/j.jphotobiol.2012.09.020
M3 - Article
C2 - 23123596
AN - SCOPUS:84868244356
VL - 117
SP - 164
EP - 170
JO - Journal of Photochemistry and Photobiology B: Biology
JF - Journal of Photochemistry and Photobiology B: Biology
SN - 1011-1344
ER -