Alpha-2-macroglobulin loaded microcapsules enhance human leukocyte functions and innate immune response

Donata Federici Canova, Anton M. Pavlov, Lucy V. Norling, Thomas Gobbetti, Sandra Brunelleschi, Pauline Le Fauder, Nicolas Cenac, Gleb B. Sukhorukov, Mauro Perretti

Результат исследований: Материалы для журналаСтатья

13 Цитирования (Scopus)

Выдержка

Synthetic microstructures can be engineered to deliver bioactive compounds impacting on their pharmacokinetics and pharmacodynamics. Herein, we applied dextran-based layer-by-layer (LbL) microcapsules to deliver alpha-2-macroglobulin (α2MG), a protein with modulatory properties in inflammation. Extending recent observations made with dextran-microcapsules loaded with α2MG in experimental sepsis, we focused on the physical and chemical characteristics of these microstructures and determined their biology on rodent and human cells. We report an efficient encapsulation of α2MG into microcapsules, which enhanced i) human leukocyte recruitment to inflamed endothelium and ii) human macrophage phagocytosis: in both settings microcapsules were more effective than soluble α2MG or empty microcapsules (devoid of active protein). Translation of these findings revealed that intravenous administration of α2MG-microcapsules (but not empty microcapsules) promoted neutrophil migration into peritoneal exudates and augmented macrophage phagocytic functions, the latter response being associated with alteration of bioactive lipid mediators as assessed by mass spectrometry. The present study indicates that microencapsulation can be an effective strategy to harness the complex biology of α2MG with enhancing outcomes on fundamental processes of the innate immune response paving the way to potential future development in the control of sepsis.

Язык оригиналаАнглийский
Страницы (с-по)284-292
Число страниц9
ЖурналJournal of Controlled Release
Том217
DOI
СостояниеОпубликовано - 10 ноя 2015
Опубликовано для внешнего пользованияДа

Отпечаток

alpha-Macroglobulins
Innate Immunity
Capsules
Macroglobulins
Leukocytes
Dextrans
Sepsis
Macrophages
Drug Compounding
Exudates and Transudates
Phagocytosis
Intravenous Administration
Endothelium
Rodentia
Mass Spectrometry
Proteins
Neutrophils
Pharmacokinetics
Inflammation
Lipids

ASJC Scopus subject areas

  • Pharmaceutical Science

Цитировать

Canova, D. F., Pavlov, A. M., Norling, L. V., Gobbetti, T., Brunelleschi, S., Le Fauder, P., ... Perretti, M. (2015). Alpha-2-macroglobulin loaded microcapsules enhance human leukocyte functions and innate immune response. Journal of Controlled Release, 217, 284-292. https://doi.org/10.1016/j.jconrel.2015.09.021

Alpha-2-macroglobulin loaded microcapsules enhance human leukocyte functions and innate immune response. / Canova, Donata Federici; Pavlov, Anton M.; Norling, Lucy V.; Gobbetti, Thomas; Brunelleschi, Sandra; Le Fauder, Pauline; Cenac, Nicolas; Sukhorukov, Gleb B.; Perretti, Mauro.

В: Journal of Controlled Release, Том 217, 10.11.2015, стр. 284-292.

Результат исследований: Материалы для журналаСтатья

Canova, DF, Pavlov, AM, Norling, LV, Gobbetti, T, Brunelleschi, S, Le Fauder, P, Cenac, N, Sukhorukov, GB & Perretti, M 2015, 'Alpha-2-macroglobulin loaded microcapsules enhance human leukocyte functions and innate immune response', Journal of Controlled Release, том. 217, стр. 284-292. https://doi.org/10.1016/j.jconrel.2015.09.021
Canova DF, Pavlov AM, Norling LV, Gobbetti T, Brunelleschi S, Le Fauder P и соавт. Alpha-2-macroglobulin loaded microcapsules enhance human leukocyte functions and innate immune response. Journal of Controlled Release. 2015 Нояб. 10;217:284-292. https://doi.org/10.1016/j.jconrel.2015.09.021
Canova, Donata Federici ; Pavlov, Anton M. ; Norling, Lucy V. ; Gobbetti, Thomas ; Brunelleschi, Sandra ; Le Fauder, Pauline ; Cenac, Nicolas ; Sukhorukov, Gleb B. ; Perretti, Mauro. / Alpha-2-macroglobulin loaded microcapsules enhance human leukocyte functions and innate immune response. В: Journal of Controlled Release. 2015 ; Том 217. стр. 284-292.
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AU - Brunelleschi, Sandra

AU - Le Fauder, Pauline

AU - Cenac, Nicolas

AU - Sukhorukov, Gleb B.

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