A plasmid vector with positive selection and directional cloning based on a conditionally lethal gene

Sergey A. Yazynin, Sergey M. Deyev, Milan Jucovič, Robert W. Hartley

    Результат исследований: Материалы для журналаСтатья

    22 Цитирования (Scopus)

    Выдержка

    A plasmid vector with a multiple cloning site (MCS) for positive selection of cloned inserts in Escherichia coli (Ec) has been devised, based on the expression plasmid (pMT416) for the bacterial ribonuclease barnase (Barn). The host is protected from the lethal effect of moderate expression of barn by expression of the gene bars, encoding its inhibitor, barstar (Bars), placed on the same plasmid. Full expression, however, is lethal. Induction is also lethal with the derived plasmid, pMT440, which has the pUC19 MCS inserted into barn. Under inducing conditions, transformation by the vector is lethal unless the product of the modified barn is inactivated by insertion of cloned DNA fragments into the MCS. Plasmid pMT440 is, therefore, a generally useful selective cloning vector not requiring any special strain of Ec.

    Язык оригиналаАнглийский
    Страницы (с-по)131-132
    Число страниц2
    ЖурналGene
    Том169
    Номер выпуска1
    DOI
    СостояниеОпубликовано - 22 фев 1996

    Отпечаток

    Lethal Genes
    Organism Cloning
    Plasmids
    Escherichia coli
    Genetic Vectors
    Ribonucleases
    Gene Expression
    DNA

    ASJC Scopus subject areas

    • Medicine(all)
    • Genetics

    Цитировать

    A plasmid vector with positive selection and directional cloning based on a conditionally lethal gene. / Yazynin, Sergey A.; Deyev, Sergey M.; Jucovič, Milan; Hartley, Robert W.

    В: Gene, Том 169, № 1, 22.02.1996, стр. 131-132.

    Результат исследований: Материалы для журналаСтатья

    Yazynin, Sergey A. ; Deyev, Sergey M. ; Jucovič, Milan ; Hartley, Robert W. / A plasmid vector with positive selection and directional cloning based on a conditionally lethal gene. В: Gene. 1996 ; Том 169, № 1. стр. 131-132.
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    abstract = "A plasmid vector with a multiple cloning site (MCS) for positive selection of cloned inserts in Escherichia coli (Ec) has been devised, based on the expression plasmid (pMT416) for the bacterial ribonuclease barnase (Barn). The host is protected from the lethal effect of moderate expression of barn by expression of the gene bars, encoding its inhibitor, barstar (Bars), placed on the same plasmid. Full expression, however, is lethal. Induction is also lethal with the derived plasmid, pMT440, which has the pUC19 MCS inserted into barn. Under inducing conditions, transformation by the vector is lethal unless the product of the modified barn is inactivated by insertion of cloned DNA fragments into the MCS. Plasmid pMT440 is, therefore, a generally useful selective cloning vector not requiring any special strain of Ec.",
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    AU - Yazynin, Sergey A.

    AU - Deyev, Sergey M.

    AU - Jucovič, Milan

    AU - Hartley, Robert W.

    PY - 1996/2/22

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    N2 - A plasmid vector with a multiple cloning site (MCS) for positive selection of cloned inserts in Escherichia coli (Ec) has been devised, based on the expression plasmid (pMT416) for the bacterial ribonuclease barnase (Barn). The host is protected from the lethal effect of moderate expression of barn by expression of the gene bars, encoding its inhibitor, barstar (Bars), placed on the same plasmid. Full expression, however, is lethal. Induction is also lethal with the derived plasmid, pMT440, which has the pUC19 MCS inserted into barn. Under inducing conditions, transformation by the vector is lethal unless the product of the modified barn is inactivated by insertion of cloned DNA fragments into the MCS. Plasmid pMT440 is, therefore, a generally useful selective cloning vector not requiring any special strain of Ec.

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